ID   VF199_ASFP4             Reviewed;         199 AA.
AC   P0CA95;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=Inner membrane protein E199L {ECO:0000305};
DE            Short=pE199L;
GN   OrderedLocusNames=Pret-142;
OS   African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=561443;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential for viral fusion with host endosomal membrane and
CC       core release (By similarity). Not required for virus morphogenesis and
CC       egress (By similarity). Induces complete autophagy through the
CC       interaction with and down-regulation of host PYCR2 (By similarity).
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SUBUNIT: Interacts with host PYCR2; this interaction results in
CC       autophagy activation. {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:Q65198}.
CC       Host membrane {ECO:0000305}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q65198}. Note=Found in the perinuclear
CC       cytoplasmic viral factories during assembly (By similarity). Part of
CC       the virion inner membrane (By similarity).
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- PTM: Contains intramolecular disulfide bonds.
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SIMILARITY: Belongs to the asfivirus E199L family. {ECO:0000305}.
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DR   EMBL; AY261363; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; P0CA95; -.
DR   Proteomes; UP000000859; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039520; P:induction by virus of host autophagy; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Activation of host autophagy by virus; Disulfide bond; Glycoprotein;
KW   Host membrane; Host-virus interaction; Late protein; Membrane;
KW   Transmembrane; Transmembrane helix; Virion.
FT   CHAIN           1..199
FT                   /note="Inner membrane protein E199L"
FT                   /id="PRO_0000373584"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        131
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   199 AA;  22010 MW;  544E191839C5DF03 CRC64;
     MSCMPVSTKC NDIWVDFSCT GPSISELQKK EPKAWAAILR SHTNQQTAED DTIIGSICDK
     QGLCSKDEYA YSQYCACVNS GTLWAECAFA PCNGNKNAYK TTEQRNILTN KQCPSGLTIC
     QNIAEYGGSG NISDLYQNFN CNSVINTFLI NVMNHPFLTL ILIILILIII YRLMSSSGGK
     HNDDKLPPPS LIFSNLNNF