ID   VF199_ASFWA             Reviewed;         200 AA.
AC   P0CA96;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 26.
DE   RecName: Full=Inner membrane protein E199L {ECO:0000305};
DE            Short=pE199L;
GN   OrderedLocusNames=War-140;
OS   African swine fever virus (isolate Warthog/Namibia/Wart80/1980) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=561444;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential for viral fusion with host endosomal membrane and
CC       core release (By similarity). Not required for virus morphogenesis and
CC       egress (By similarity). Induces complete autophagy through the
CC       interaction with and down-regulation of host PYCR2 (By similarity).
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SUBUNIT: Interacts with host PYCR2; this interaction results in
CC       autophagy activation. {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:Q65198}.
CC       Host membrane {ECO:0000305}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q65198}. Note=Found in the perinuclear
CC       cytoplasmic viral factories during assembly (By similarity). Part of
CC       the virion inner membrane (By similarity).
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- PTM: Contains intramolecular disulfide bonds.
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SIMILARITY: Belongs to the asfivirus E199L family. {ECO:0000305}.
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DR   EMBL; AY261366; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; P0CA96; -.
DR   Proteomes; UP000000858; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039520; P:induction by virus of host autophagy; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Activation of host autophagy by virus; Disulfide bond; Glycoprotein;
KW   Host membrane; Host-virus interaction; Late protein; Membrane;
KW   Transmembrane; Transmembrane helix; Virion.
FT   CHAIN           1..200
FT                   /note="Inner membrane protein E199L"
FT                   /id="PRO_0000373585"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        131
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   200 AA;  22115 MW;  A9CA068117FA779B CRC64;
     MSCMPVSTKC NDIWVDFSCT GPSISELQKK EPKAWAAIVR SRTNQQTAED DNIIGSICDK
     QGLCSKDEYA YSQYCACVNS GTLWAECAFA PCNGNKNAYK TTEQRNILTN KQCPSGLTIC
     QNIAEYGGSG NISDLYQNFN CNSVINTFLI NVMNHPFLTL ILIILILIII YRLMSSSSGG
     KHNDDKLPPP SLIFSNLNNF