ID   VF424_ASFB7             Reviewed;         424 AA.
AC   Q65148;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Probable methyltransferase EP424R;
DE            Short=pEP424R;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=Ba71V-055; ORFNames=EP424R;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=30185597; DOI=10.1128/jvi.01293-18;
RA   Alejo A., Matamoros T., Guerra M., Andres G.;
RT   "A Proteomic Atlas of the African Swine Fever Virus Particle.";
RL   J. Virol. 92:0-0(2018).
RN   [3]
RP   INDUCTION.
RX   PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA   Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA   Dixon L., Werner F.;
RT   "The African Swine Fever Virus Transcriptome.";
RL   J. Virol. 94:0-0(2020).
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:30185597}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:32075923}.
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DR   EMBL; U18466; AAA65285.1; -; Genomic_DNA.
DR   RefSeq; NP_042749.1; NC_001659.2.
DR   SMR; Q65148; -.
DR   GeneID; 22220437; -.
DR   KEGG; vg:22220437; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   InterPro; IPR025807; Adrift-typ_MeTrfase.
DR   InterPro; IPR002877; RNA_MeTrfase_FtsJ_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF01728; FtsJ; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51614; SAM_MT_ADRIFT; 1.
PE   2: Evidence at transcript level;
KW   Early protein; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase; Virion.
FT   CHAIN           1..424
FT                   /note="Probable methyltransferase EP424R"
FT                   /id="PRO_0000373065"
FT   DOMAIN          104..316
FT                   /note="Adrift-type SAM-dependent 2'-O-MTase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
FT   ACT_SITE        269
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
FT   BINDING         136
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
FT   BINDING         229
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
SQ   SEQUENCE   424 AA;  49332 MW;  8BFB35EFF666EA0E CRC64;
     MSNYYYYYGG GRYDWLKTVE PTNFLKIGLP YQAHPLHLQH QATTTPPSIL EKFKRADILL
     NEVKAEMDPL MLQPETEKKL YQILGSIDMF KGLRKKVEFT YNAQIVTNAW LKMYELLNTM
     NFNNTSQAFC NCELPGGFIS AINHFNYTMM HYPTFNWVAS SLYPSSETDA LEDHYGLYQC
     NPDNWLMQSP LLKKNVDYND GDVTIASNVK NLALRATQRL TPIHLYTADG GINVGHDYNK
     QEELNLKLHF GQALTGLLSL SKGGNMILKH YTLNHAFTLS LICVFSHFFE ELYITKPTSS
     RPTNSETYIV GKNRLRLFTP KEEQILLKRL EFFNDTPLVD LSLYQNLLES IYFAVETIHL
     KQQIEFLNFG MKCYRHFYNK IKLLNEYLAP KKKIFQDRWR VLNKLYVLEK KHKLKLCAPQ
     GSVA