ID   VF424_ASFK5             Reviewed;         424 AA.
AC   P0C968;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 26.
DE   RecName: Full=Probable methyltransferase EP424R;
DE            Short=pEP424R;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=Ken-067;
OS   African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=561445;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:Q65148}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000305}.
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DR   EMBL; AY261360; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; P0C968; -.
DR   Proteomes; UP000000861; Genome.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   InterPro; IPR025807; Adrift-typ_MeTrfase.
DR   InterPro; IPR002877; RNA_MeTrfase_FtsJ_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF01728; FtsJ; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51614; SAM_MT_ADRIFT; 1.
PE   3: Inferred from homology;
KW   Early protein; Methyltransferase; S-adenosyl-L-methionine; Transferase;
KW   Virion.
FT   CHAIN           1..424
FT                   /note="Probable methyltransferase EP424R"
FT                   /id="PRO_0000373067"
FT   DOMAIN          103..315
FT                   /note="Adrift-type SAM-dependent 2'-O-MTase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
FT   ACT_SITE        268
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
FT   BINDING         135
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
FT   BINDING         228
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
SQ   SEQUENCE   424 AA;  49337 MW;  ACE65A3A08A6B479 CRC64;
     MSNYYYYYGG GRYDWLKTVE PTNFLKIGLP YQAHPLHLQH QATTPPSILE KFKRADILLN
     EVKSEMDPLM LQPETEKKLY QVLGSIDMFK GLRKKVEYTY NAQIVTNAWL KMYELLNTMN
     FNNTSQAFCN CELPGGFISA INHFNHTMMH YPTFNWVASS LYPSSETDAL EDHYGLYQCN
     PDNWLMQSPL LKKNMDYNNG DVTIASNVKN LALKATQKLT PIHLYTADGG INVGHDYNKQ
     EELNLKLHFG QALTGLLSLS KGGNMILKHY TLNHAFTLSL ICVFSHFFEE LYITKPTSSR
     PSNSETYIVG KNRLRLFTPK EEQVLLKRLE FFNDTPLVDL SLYQNLLESI YFAVETIHLK
     QQIEFLNFGM KCYRHFYNKI KLLNEYLAPK KKIFQDRWRV LNKLYVLEKK HKLKLCTSFQ
     GSVA