ID   VF424_ASFP4             Reviewed;         424 AA.
AC   P0C969;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 29.
DE   RecName: Full=Probable methyltransferase EP424R;
DE            Short=pEP424R;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=Pret-067;
OS   African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=561443;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:Q65148}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000305}.
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DR   EMBL; AY261363; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; P0C969; -.
DR   Proteomes; UP000000859; Genome.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   InterPro; IPR025807; Adrift-typ_MeTrfase.
DR   InterPro; IPR002877; RNA_MeTrfase_FtsJ_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF01728; FtsJ; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51614; SAM_MT_ADRIFT; 1.
PE   3: Inferred from homology;
KW   Early protein; Methyltransferase; S-adenosyl-L-methionine; Transferase;
KW   Virion.
FT   CHAIN           1..424
FT                   /note="Probable methyltransferase EP424R"
FT                   /id="PRO_0000373068"
FT   DOMAIN          103..315
FT                   /note="Adrift-type SAM-dependent 2'-O-MTase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
FT   ACT_SITE        268
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
FT   BINDING         135
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
FT   BINDING         228
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00946"
SQ   SEQUENCE   424 AA;  49295 MW;  72E89CFD57D1C2B3 CRC64;
     MSNYYYYYGG GRYDWLKTVE PTNFLKIGLP YQAHPLHLQH QATTPPSILE KFKRADILLN
     EVKAEMDPLM LQPETEKKLF QILSSIDMFK GLRKKVEFTY NAQIVTNAWL KMYELLNTMN
     FNNTSQAFCN CELPGGFISA INHFNYTMMH YPTFNWVASS LYPSSETDAL EDHYGLYQCN
     PDNWLMQSPL LKKNMDYNNG DVTIASNVKN LALRATQRLT PIHLYTADGG INVGHDYNKQ
     EELNLKLHFG QALTGLLSLS KGGNMILKHY TLNHAFTLSL ICVFSHFFEE LYITKPTSSR
     PTNSETYIVG KNRLRLFTPK EEQVLLKRLE FFNDTPLVDL SLYQNLLESV YFAVETIHLK
     QQIEFLNFGM KCYRHFYNKI KLLNDYLAPK KKIFQDRWRV LNKLYVLEKK HKLKLCAASQ
     GSVA