ID   VF69R_ASFB7             Reviewed;          69 AA.
AC   Q65134;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 60.
DE   RecName: Full=Uncharacterized membrane protein X69R;
GN   OrderedLocusNames=BA71V-018; ORFNames=X69R;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2325203; DOI=10.1128/jvi.64.5.2073-2081.1990;
RA   Gonzalez A., Calvo V., Almazan F., Almendral J.M., Ramirez J.C.,
RA   de la Vega I., Blasco R., Vinuela E.;
RT   "Multigene families in African swine fever virus: family 360.";
RL   J. Virol. 64:2073-2081(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=Georgia 2007/1;
RX   PubMed=32825617; DOI=10.3390/v12090918;
RA   Ramirez-Medina E., Vuono E., Pruitt S., Rai A., Silva E., Zhu J.,
RA   Velazquez-Salinas L., Gladue D.P., Borca M.V.;
RT   "X69R Is a Non-Essential Gene That, When Deleted from African Swine Fever,
RT   Does Not Affect Virulence in Swine.";
RL   Viruses 12:0-0(2020).
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: No effect on virus viability or replication in
CC       vitro. {ECO:0000269|PubMed:32825617}.
CC   -!- SIMILARITY: Belongs to the asfivirus X69R family. {ECO:0000305}.
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DR   EMBL; U18466; AAA65249.1; -; Genomic_DNA.
DR   EMBL; U81365; AAC40562.1; -; Genomic_DNA.
DR   RefSeq; NP_042713.1; NC_001659.2.
DR   SMR; Q65134; -.
DR   GeneID; 22220403; -.
DR   KEGG; vg:22220403; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Glycoprotein; Host membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..69
FT                   /note="Uncharacterized membrane protein X69R"
FT                   /id="PRO_0000373715"
FT   TOPO_DOM        1..15
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        37..69
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        51
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   69 AA;  8345 MW;  6192E4DB23CA7719 CRC64;
     MLLYIVIIVA CIISKLVPNE YWAIHLFFII MIFMVYMYEK LDIHQKYQFW NYTMSGLSGH
     NVQVICKCY