VFA1_YEAST
ID VFA1_YEAST Reviewed; 203 AA.
AC P40080; D3DM34;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=VPS4-associated protein 1;
GN Name=VFA1; OrderedLocusNames=YER128W; ORFNames=SYGP-ORF44;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP INTERACTION WITH VPS4, SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=21777356; DOI=10.1111/j.1600-0854.2011.01252.x;
RA Arlt H., Perz A., Ungermann C.;
RT "An overexpression screen in Saccharomyces cerevisiae identifies novel
RT genes that affect endocytic protein trafficking.";
RL Traffic 12:1592-1603(2011).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: VPS4-associated protein involved in trafficking to the
CC vacuole. {ECO:0000269|PubMed:21777356}.
CC -!- SUBUNIT: Interacts with VPS4. {ECO:0000269|PubMed:21777356}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21777356}. Endosome
CC {ECO:0000269|PubMed:21777356}. Note=Requires VPS4 for its recruitment
CC to endosomes.
CC -!- MISCELLANEOUS: Present with 967 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U18916; AAC03226.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07788.1; -; Genomic_DNA.
DR PIR; S43222; S43222.
DR RefSeq; NP_011054.3; NM_001179018.3.
DR PDB; 4NIQ; X-ray; 2.30 A; C/D=182-203.
DR PDB; 5FVK; X-ray; 1.66 A; C/D=182-203.
DR PDBsum; 4NIQ; -.
DR PDBsum; 5FVK; -.
DR AlphaFoldDB; P40080; -.
DR SMR; P40080; -.
DR BioGRID; 36872; 90.
DR DIP; DIP-5406N; -.
DR IntAct; P40080; 3.
DR STRING; 4932.YER128W; -.
DR iPTMnet; P40080; -.
DR MaxQB; P40080; -.
DR PaxDb; P40080; -.
DR PRIDE; P40080; -.
DR DNASU; 856865; -.
DR EnsemblFungi; YER128W_mRNA; YER128W; YER128W.
DR GeneID; 856865; -.
DR KEGG; sce:YER128W; -.
DR SGD; S000000930; VFA1.
DR VEuPathDB; FungiDB:YER128W; -.
DR eggNOG; ENOG502RW3H; Eukaryota.
DR HOGENOM; CLU_088285_2_1_1; -.
DR InParanoid; P40080; -.
DR OMA; KDFFYVC; -.
DR BioCyc; YEAST:G3O-30291-MON; -.
DR PRO; PR:P40080; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40080; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0001671; F:ATPase activator activity; IDA:SGD.
DR GO; GO:0032781; P:positive regulation of ATP-dependent activity; IDA:SGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007034; P:vacuolar transport; IMP:SGD.
DR InterPro; IPR013640; Vfa1.
DR PANTHER; PTHR28218; PTHR28218; 1.
DR Pfam; PF08432; Vfa1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Cytoplasm; Endosome; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..203
FT /note="VPS4-associated protein 1"
FT /id="PRO_0000202649"
FT REGION 99..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 171..193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 121..157
FT /evidence="ECO:0000255"
FT COMPBIAS 108..125
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 189..195
FT /evidence="ECO:0007829|PDB:5FVK"
SQ SEQUENCE 203 AA; 23489 MW; 819930C50DD56B1D CRC64;
MINEYVARKV ALKDMQPCAI CSKPSTTVLY NASGPDWLYT CEIHLQDNPQ FVIPLYSTEY
NEAVAQLKLV KGKMDSLTSA QTQLGSWDGW VTKIFSKKEK ETNNSKDPDP TTTDSTDTSP
QAKNDAEILS ETKKQYSKIL DKVTELQRKN RKYELAKIMF ESRLLRKRTE QVNRERYLKE
QENYSNTDPE ELLRKHVFPS VPK
