ID   VFE66_ASFB7             Reviewed;          66 AA.
AC   Q65204;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=host translation inhibitor E66L {ECO:0000305};
DE            Short=pE66L;
GN   OrderedLocusNames=Ba71V-136; ORFNames=E66L;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=33328305; DOI=10.1128/jvi.01872-20;
RA   Shen Z., Chen C., Yang Y., Xie Z., Ao Q., Lv L., Zhang S., Chen H., Hu R.,
RA   Chen H., Peng G.;
RT   "A novel function of African Swine Fever Virus pE66L in inhibition of host
RT   translation by the PKR/eIF2alpha pathway.";
RL   J. Virol. 0:0-0(2020).
CC   -!- FUNCTION: Inhibits host protein translation, probably through the
CC       EIF2AK2/EIF2S1 signaling pathway (PubMed:33328305). Promotes cell
CC       retention in the G0/G1 phase (PubMed:33328305).
CC       {ECO:0000269|PubMed:33328305}.
CC   -!- SUBCELLULAR LOCATION: Host endoplasmic reticulum
CC       {ECO:0000269|PubMed:33328305}.
CC   -!- SIMILARITY: Belongs to the asfivirus E66L family. {ECO:0000305}.
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DR   EMBL; U18466; AAA65364.1; -; Genomic_DNA.
DR   RefSeq; NP_042828.1; NC_001659.2.
DR   GeneID; 22220364; -.
DR   KEGG; vg:22220364; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0044165; C:host cell endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW.
DR   GO; GO:0039657; P:suppression by virus of host gene expression; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Eukaryotic host gene expression shutoff by virus;
KW   Eukaryotic host translation shutoff by virus;
KW   G0/G1 host cell cycle checkpoint dysregulation by virus;
KW   Host endoplasmic reticulum; Host gene expression shutoff by virus;
KW   Host-virus interaction; Modulation of host cell cycle by virus;
KW   Reference proteome.
FT   CHAIN           1..66
FT                   /note="host translation inhibitor E66L"
FT                   /id="PRO_0000373766"
FT   REGION          13..34
FT                   /note="Involved in the inhibition of host protein
FT                   synthesis"
FT                   /evidence="ECO:0000269|PubMed:33328305"
SQ   SEQUENCE   66 AA;  7936 MW;  A3DC19CA5262F7F2 CRC64;
     MLTMVSLARF IINTASFNKS IYPLSQVYVK IHLSHKYNHI LFTYNMRIYL IKRNHMLFTH
     MLISCN