ID   VFUS_VACC6              Reviewed;         136 AA.
AC   P26312;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=14 kDa fusion protein;
GN   ORFNames=A27L;
OS   Vaccinia virus (strain WR 65-16) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10247;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2389560; DOI=10.1016/0042-6822(90)90381-z;
RA   Gong S., Lai C., Esteban M.;
RT   "Vaccinia virus induces cell fusion at acid pH and this activity is
RT   mediated by the N-terminus of the 14-kDa virus envelope protein.";
RL   Virology 178:81-91(1990).
CC   -!- FUNCTION: This protein appears to play an important role in virus
CC       penetration at the level of cell fusion. The N-terminal proximal region
CC       is essential for fusion ability. Essential in fusing the outermost of
CC       the two Golgi-derived membranes enveloping the virus with the plasma
CC       membrane, and in its subsequent release extracellularly.
CC   -!- SUBUNIT: Homotrimer, covalently linked.
CC   -!- SUBCELLULAR LOCATION: Virion membrane. Note=Envelope fraction of
CC       virions.
CC   -!- SIMILARITY: Belongs to the poxviruses fusion protein family.
CC       {ECO:0000305}.
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DR   EMBL; M37086; AAA47961.1; -; Genomic_DNA.
DR   PIR; A37076; WMVZ65.
DR   SMR; P26312; -.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:InterPro.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:InterPro.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR003436; Chordopox_Fusion/A27.
DR   Pfam; PF02346; Vac_Fusion; 1.
DR   PRINTS; PR01847; VIRALFUSION.
PE   3: Inferred from homology;
KW   Glycoprotein; Host-virus interaction; Membrane;
KW   Viral attachment to host cell; Virion; Virus entry into host cell.
FT   CHAIN           1..136
FT                   /note="14 kDa fusion protein"
FT                   /id="PRO_0000099213"
FT   REGION          22..50
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        22..38
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        86
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   136 AA;  15837 MW;  5F685204E808D108 CRC64;
     MLEFFRPPRA RSPRELVQLL PEAWTTSRSS TGSANPSASR KPARYPRIHA PELQSGEARW
     PLWSRIRPLE DPLKQRLTNL EKKITNVTTK FEQIEKCCKR NDEVLFRLEN HAETLRAAMI
     SLAKKIDVQT GRRPYE