ID   VG08_BPP22              Reviewed;         303 AA.
AC   P26748; Q7PCI6;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   02-JUN-2021, entry version 83.
DE   RecName: Full=Scaffolding protein;
DE   AltName: Full=Protein gp8;
GN   Name=8;
OS   Salmonella phage P22 (Bacteriophage P22).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Podoviridae; Lederbergvirus.
OX   NCBI_TaxID=10754;
OH   NCBI_TaxID=90371; Salmonella typhimurium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15.
RX   PubMed=1853558; DOI=10.1016/0042-6822(91)90981-g;
RA   Eppler K., Wyckoff E., Goates J., Parr R., Casjens S.;
RT   "Nucleotide sequence of the bacteriophage P22 genes required for DNA
RT   packaging.";
RL   Virology 183:519-538(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11053393; DOI=10.1128/jb.182.22.6472-6481.2000;
RA   Vander Byl C.S., Kropinski A.M.B.;
RT   "Sequence of the genome of Salmonella bacteriophage P22.";
RL   J. Bacteriol. 182:6472-6481(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12562822; DOI=10.1128/jb.185.4.1475-1477.2003;
RA   Pedulla M.L., Ford M.E., Karthikeyan T., Houtz J.M., Hendrix R.W.,
RA   Hatfull G.F., Poteete A.R., Gilcrease E.B., Winn-Stapley D.A.,
RA   Casjens S.R.;
RT   "Corrected sequence of the bacteriophage P22 genome.";
RL   J. Bacteriol. 185:1475-1477(2003).
CC   -!- FUNCTION: Required for successful condensation of DNA within the
CC       capsid. The interior of the prohead is filled with the gp8 protein. The
CC       scaffolding protein is lost from the structure during packaging.
CC   -!- INDUCTION: The scaffolding protein negatively regulates its own
CC       synthesis when it is not assembled into proheads.
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DR   EMBL; M59749; AAA72962.1; -; Genomic_DNA.
DR   EMBL; AF217253; AAF75046.1; -; Genomic_DNA.
DR   EMBL; BK000583; DAA00986.1; -; Genomic_DNA.
DR   PIR; D40474; Z8BP22.
DR   RefSeq; YP_063736.1; NC_002371.2.
DR   PDB; 1GP8; NMR; -; A=264-303.
DR   PDB; 2GP8; NMR; -; A=264-303.
DR   PDBsum; 1GP8; -.
DR   PDBsum; 2GP8; -.
DR   BMRB; P26748; -.
DR   SMR; P26748; -.
DR   GeneID; 2944242; -.
DR   KEGG; vg:2944242; -.
DR   EvolutionaryTrace; P26748; -.
DR   Proteomes; UP000001795; Genome.
DR   Proteomes; UP000007960; Genome.
DR   Gene3D; 4.10.810.10; -; 1.
DR   InterPro; IPR015385; Phage_P22_Gp8_scaffold.
DR   InterPro; IPR027393; Virus_scaffolding_prot_C.
DR   Pfam; PF09306; Phage-scaffold; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Late protein; Reference proteome.
FT   CHAIN           1..303
FT                   /note="Scaffolding protein"
FT                   /id="PRO_0000077756"
FT   HELIX           269..284
FT                   /evidence="ECO:0007829|PDB:1GP8"
FT   HELIX           289..299
FT                   /evidence="ECO:0007829|PDB:1GP8"
SQ   SEQUENCE   303 AA;  33565 MW;  B86BD625D5011ABE CRC64;
     MEPTTEIQAT EDLTLSGDHA AASADSLVVD NANDNAGQEE GFEIVLKDDE TAPKQDPAKN
     AEFARRRIER KRQRELEQQM EAVKRGELPE SLRVNPDLPP QPDINAYLSE EGLAKYDYDN
     SRALAAFNAA NTEWLMKAQD ARSNAVAEQG RKTQEFTQQS AQYVEAARKH YDAAEKLNIP
     DYQEKEDAFM QLVPPAVGAD IMRLFPEKSA ALMYHLGANP EKARQLLAMD GQSALIELTR
     LSERLTLKPR GKQISSAPPA DQPITGDVSA ANKDAIRKQM DAAASKGDVE TYRKLKAKLK
     GIR