ID   VG4_BPNF                Reviewed;         125 AA.
AC   P09877;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Late genes activator;
DE   AltName: Full=Early protein GP4;
DE   AltName: Full=GPF;
GN   Name=4; Synonyms=F;
OS   Bacillus phage Nf (Bacteriophage Nf).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Salasmaviridae; Picovirinae; Beecentumtrevirus.
OX   NCBI_TaxID=10753;
OH   NCBI_TaxID=1423; Bacillus subtilis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3015737; DOI=10.1016/0378-1119(86)90302-1;
RA   Mizukami Y., Sekiya T., Hirokawa H.;
RT   "Nucleotide sequence of gene F of Bacillus phage Nf.";
RL   Gene 42:231-235(1986).
CC   -!- FUNCTION: This protein is believed to be a positive regulator of late
CC       transcription. It may function as a sigma-like component of the host
CC       RNA polymerase. Binds to a region of the A3 promoter located between
CC       nucleotides -50 and -100 relative to the transcription start site, that
CC       presents a sequence-directed curvature. Full induction of this
CC       curvature is needed for the transcription activation process (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the podoviruses GP4 family. {ECO:0000305}.
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DR   EMBL; M13664; AAA32195.1; -; Genomic_DNA.
DR   PIR; A25643; ERBPNF.
DR   SMR; P09877; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.3560; -; 1.
DR   InterPro; IPR038246; Phi-29-like_sf.
DR   InterPro; IPR008771; Phi-29_GP4.
DR   Pfam; PF05464; Phi-29_GP4; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-directed RNA polymerase; Early protein;
KW   Nucleotidyltransferase; Sigma factor; Transcription;
KW   Transcription regulation; Transferase.
FT   CHAIN           1..125
FT                   /note="Late genes activator"
FT                   /id="PRO_0000106559"
FT   DNA_BIND        77..96
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   125 AA;  15019 MW;  DBC8AD3EC41A643D CRC64;
     MPRTARGIYH NLKESEYVVS NGDATFFFFS EMYQNKFLDG YQKHREEFNK KINRITDTPL
     NMDMLADITF YSNVEKRGFH AWLKGVNTTW QEIHVYALRT MTKPCTQNWS RIRKPKLVER
     RKSMV