ID   VG71_BPML5              Reviewed;         183 AA.
AC   Q05286;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   23-FEB-2022, entry version 73.
DE   RecName: Full=Repressor-like immunity protein;
DE   AltName: Full=Gp71;
GN   Name=71; Synonyms=IMM;
OS   Mycobacterium phage L5 (Mycobacteriophage L5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Fromanvirus.
OX   NCBI_TaxID=31757;
OH   NCBI_TaxID=1763; Mycobacterium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8459766; DOI=10.1111/j.1365-2958.1993.tb01131.x;
RA   Hatfull G.F., Sarkis G.J.;
RT   "DNA sequence, structure and gene expression of mycobacteriophage L5: a
RT   phage system for mycobacterial genetics.";
RL   Mol. Microbiol. 7:395-405(1993).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=8459767; DOI=10.1111/j.1365-2958.1993.tb01132.x;
RA   Donnelly-Wu M.K., Jacobs W.R. Jr., Hatfull G.F.;
RT   "Superinfection immunity of mycobacteriophage L5: applications for genetic
RT   transformation of mycobacteria.";
RL   Mol. Microbiol. 7:407-417(1993).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=9312049; DOI=10.1093/emboj/16.19.5914;
RA   Brown K.L., Sarkis G.J., Wadsworth C., Hatfull G.F.;
RT   "Transcriptional silencing by the mycobacteriophage L5 repressor.";
RL   EMBO J. 16:5914-5921(1997).
CC   -!- FUNCTION: Confers immunity to L5 superinfection, required for
CC       maintenance of the lysogenic state. Binds to multiple asymmetric DNA
CC       sites. Regulates transcription initiation at an early lytic promoter,
CC       Pleft, but also affects downstream gene expression at 'stoperator'
CC       sites in the phage genome.
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DR   EMBL; Z18946; CAA79447.1; -; Genomic_DNA.
DR   PIR; S31016; S31016.
DR   RefSeq; NP_039735.1; NC_001335.1.
DR   GeneID; 2942987; -.
DR   KEGG; vg:2942987; -.
DR   Proteomes; UP000002123; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..183
FT                   /note="Repressor-like immunity protein"
FT                   /id="PRO_0000164814"
FT   DNA_BIND        34..53
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         108
FT                   /note="R->L: Class I phenotype."
FT   MUTAGEN         123
FT                   /note="L->P: Thermo-inducible phenotype."
FT   MUTAGEN         124
FT                   /note="E->G: Thermo-inducible phenotype."
SQ   SEQUENCE   183 AA;  21409 MW;  3D25D4FCB4806C8C CRC64;
     MSGKIQHKAV VPAPSRIPLT LSEIEDLRRK GFNQTEIAEL YGVTRQAVSW HKKTYGGRLT
     TRQIVQQNWP WDTRKPHDKS KAFQRLRDHG EYMRVGSFRT MSEDKKKRLL SWWKMLRDDD
     LVLEFDPSIE PYEGMAGGGF RYVPRGIEDD DLLIRVNEHT NLTAEGELLW SWPDDIEELL
     SEP