ID   VGA_BPAL3               Reviewed;         494 AA.
AC   P25243;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 2.
DT   23-FEB-2022, entry version 74.
DE   RecName: Full=A protein;
DE   AltName: Full=GPA;
GN   Name=A;
OS   Escherichia phage alpha3 (Bacteriophage alpha-3).
OC   Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes;
OC   Petitvirales; Microviridae; Bullavirinae; Alphatrevirus.
OX   NCBI_TaxID=10849;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1532908; DOI=10.1016/0167-4781(92)90440-b;
RA   Kodaira K., Nakano K., Okada S., Taketo A.;
RT   "Nucleotide sequence of the genome of the bacteriophage alpha 3:
RT   interrelationship of the genome structure and the gene products with those
RT   of the phages, phi X174, G4 and phi K.";
RL   Biochim. Biophys. Acta 1130:277-288(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 228-257.
RX   PubMed=2785817; DOI=10.1016/0167-4781(89)90180-2;
RA   Kodaira K., Miyata T., Suzuki K., Nakano K., Taketo A.;
RT   "Possible finger structure in gene A protein of Microviridae.";
RL   Biochim. Biophys. Acta 1008:123-124(1989).
CC   -!- FUNCTION: The A protein is a specific endonuclease that cleaves the
CC       viral strand of supertwisted, closed circular DNA at a unique site in
CC       the A gene. The A protein also causes relaxation of supertwisted DNA
CC       and forms a complex with viral DNA that has a discontinuity in gene A
CC       of the viral strand.
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DR   EMBL; X60322; CAA42874.1; -; Genomic_DNA.
DR   EMBL; X12611; CAA31130.1; -; Genomic_DNA.
DR   PIR; S22324; S22324.
DR   RefSeq; NP_039590.1; NC_001330.1.
DR   GeneID; 1260690; -.
DR   KEGG; vg:1260690; -.
DR   Proteomes; UP000002137; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   InterPro; IPR008766; Replication_gene_A.
DR   Pfam; PF05840; Phage_GPA; 1.
PE   4: Predicted;
KW   DNA replication; DNA-binding; Endonuclease; Hydrolase; Metal-binding;
KW   Nuclease; Reference proteome; Viral DNA replication; Zinc; Zinc-finger.
FT   CHAIN           1..494
FT                   /note="A protein"
FT                   /id="PRO_0000164865"
FT   ZN_FING         230..252
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        327
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        331
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   494 AA;  57368 MW;  002FCD85A4C11699 CRC64;
     MATEIFSDVV KSVWSHATAK HAHLRMAVQV DNRSRLLSDL VYQLEKRLSE ETHLDDETLA
     IYESQTALLE DHMALVRKCA AQLDNSNTID HRTPLDAECV FLFEMICPGV RYGKTLNSLW
     TKYVVQWPEK LIRQELDMVK PLSFKDEVAK YMEKMQEKTR KNRMTQKVIN EMRIAHQKGW
     FFVFDTLTLA DDRLQAFNEN PNALRDYFRT VGRAVLRAEG RSVKDSYNDC YRYLCVPEFG
     GQHGRLHWHV VHMVRTLPLG SHDPNFGRKV RNYRQINSFR GMWPYGFTQP IAVRYQHDAY
     SRKGWLWPVD KTGKAMQSKP YQAVAWYVTK YVAKQSDQRQ KAITERQKKC KNPLMAICLK
     KEFRVRSSRK LGMELPSMAH LSNKVLLELS RISFDSSPLY QIVKENAKKQ LTLNIGALPL
     HVILDVRPEV RSMLKTIRRL MKKTPEFNWQ SSIASMTVTL KNGDISDEAR QYIIDAGITP
     TDLRAKATQT FGGK