ID   VGA_BPS13               Reviewed;         522 AA.
AC   P07928;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=A' protein;
DE   AltName: Full=GPA;
GN   Name=A;
OS   Enterobacteria phage S13 (Bacteriophage S13).
OC   Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes;
OC   Petitvirales; Microviridae; Bullavirinae; Sinsheimervirus;
OC   unclassified Sinsheimervirus.
OX   NCBI_TaxID=10844;
OH   NCBI_TaxID=590; Salmonella.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3007293; DOI=10.1016/0378-1119(85)90050-2;
RA   Lau P.C.K., Spencer J.H.;
RT   "Nucleotide sequence and genome organization of bacteriophage S13 DNA.";
RL   Gene 40:273-284(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 269-333.
RX   PubMed=909772; DOI=10.1093/nar/4.7.2235;
RA   Grosveld F.G., Spencer J.H.;
RT   "The nucleotide sequence of two restriction fragments located in the gene
RT   AB region of bacteriophage S13.";
RL   Nucleic Acids Res. 4:2235-2252(1977).
CC   -!- FUNCTION: The A protein is a specific endonuclease that cleaves the
CC       viral strand of supertwisted, closed circular DNA at a unique site in
CC       the A gene. The A protein also causes relaxation of supertwisted DNA
CC       and forms a complex with viral DNA that has a discontinuity in gene A
CC       of the viral strand.
CC   -!- FUNCTION: The A* protein binds to double-stranded DNA and prevents
CC       their hydrolysis by nucleases.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=3;
CC       Name=A';
CC         IsoId=P07928-1; Sequence=Displayed;
CC       Name=A;
CC         IsoId=P07928-2; Sequence=VSP_018675;
CC       Name=A*;
CC         IsoId=P07928-3; Sequence=VSP_018676;
CC   -!- MISCELLANEOUS: [Isoform A]: Produced by alternative initiation at Met-
CC       10 of isoform A'. {ECO:0000305}.
CC   -!- MISCELLANEOUS: [Isoform A*]: Produced by alternative initiation at Met-
CC       173 of isoform A'. {ECO:0000305}.
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DR   EMBL; M14428; AAA32581.1; -; Genomic_DNA.
DR   EMBL; M14428; AAA32582.1; -; Genomic_DNA.
DR   EMBL; M14428; AAA32583.1; -; Genomic_DNA.
DR   PIR; JS0450; JS0450.
DR   Proteomes; UP000002129; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   InterPro; IPR008766; Replication_gene_A.
DR   Pfam; PF05840; Phage_GPA; 1.
PE   4: Predicted;
KW   Alternative initiation; DNA replication; DNA-binding; Endonuclease;
KW   Hydrolase; Metal-binding; Nuclease; Reference proteome;
KW   Viral DNA replication; Zinc; Zinc-finger.
FT   CHAIN           1..522
FT                   /note="A' protein"
FT                   /id="PRO_0000003319"
FT   ZN_FING         255..277
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        352
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        356
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         1..181
FT                   /note="Missing (in isoform A*)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018676"
FT   VAR_SEQ         1..9
FT                   /note="Missing (in isoform A)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018675"
SQ   SEQUENCE   522 AA;  59882 MW;  CE9DE9AC679403A2 CRC64;
     MPPNFGGFFM VRSYYPSECH ADYFDFERIE ALKPAIEACG ISTLSQSPML GFHKQMDNRI
     KLLEEILSFR MQGVEFDNGD MYVYGHKAAS DVRDEFVSVT EKLMDELAQC YNVLPQLDIN
     NTIDHPPEGD EKWFLENEKT VTQFCRKLAA ERPLKDIRDE YNYPKKKGIK DECSRLLEAS
     TMKSRRGFTI QRLMNAMRQA HADGWFIVFD TLTLADDRLE AFYDNPNALR DYFRDIGRMV
     LAAERRKAND SHADCYQYFC VPEYGTANGR LHFHAVHFMR TLPSGSVDPN FGRRVRNRRQ
     LNSLQNTWPY GYSMPIAVRY TQDAFSRSGW LWPVDAKGEP LKATSYMAVG FYVAKYVNKK
     SDMDLAAKGL GAKEWNNSLK TKLSLLPKKL FRIRMSRNFG MKMFTMTNLS MECLIQLTKL
     GYDATPFNQI LKQNAKREMR LRLGKVTVAD VLAAQPVTTN LLKFMRASIK MIGVSNLQSF
     IASMTQKLTL SDISDESKNY LDKAGITTAC LRIKSKWTAG GK