VGB_BACLD
ID VGB_BACLD Reviewed; 295 AA.
AC Q62ZD8; Q65NZ1;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Virginiamycin B lyase {ECO:0000255|HAMAP-Rule:MF_01282};
DE EC=4.2.99.- {ECO:0000255|HAMAP-Rule:MF_01282};
DE AltName: Full=Streptogramin B lyase {ECO:0000255|HAMAP-Rule:MF_01282};
GN Name=vgb {ECO:0000255|HAMAP-Rule:MF_01282};
GN OrderedLocusNames=BLi00263, BL01635;
OS Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 /
OS NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=279010;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX PubMed=15383718; DOI=10.1159/000079829;
RA Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P.,
RA Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.;
RT "The complete genome sequence of Bacillus licheniformis DSM13, an organism
RT with great industrial potential.";
RL J. Mol. Microbiol. Biotechnol. 7:204-211(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX PubMed=15461803; DOI=10.1186/gb-2004-5-10-r77;
RA Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J.,
RA Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B.,
RA Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A.,
RA Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M.;
RT "Complete genome sequence of the industrial bacterium Bacillus
RT licheniformis and comparisons with closely related Bacillus species.";
RL Genome Biol. 5:R77.1-R77.12(2004).
CC -!- FUNCTION: Inactivates the type B streptogramin antibiotics by
CC linearizing the lactone ring at the ester linkage, generating a free
CC phenylglycine carboxylate and converting the threonyl moiety into 2-
CC amino-butenoic acid. {ECO:0000255|HAMAP-Rule:MF_01282}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01282};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_01282}.
CC -!- SIMILARITY: Belongs to the Vgb family. {ECO:0000255|HAMAP-
CC Rule:MF_01282}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAU39223.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE017333; AAU39223.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP000002; AAU21870.1; -; Genomic_DNA.
DR RefSeq; WP_011197502.1; NC_006322.1.
DR AlphaFoldDB; Q62ZD8; -.
DR SMR; Q62ZD8; -.
DR STRING; 279010.BL01635; -.
DR EnsemblBacteria; AAU21870; AAU21870; BL01635.
DR GeneID; 66217579; -.
DR KEGG; bld:BLi00263; -.
DR KEGG; bli:BL01635; -.
DR PATRIC; fig|279010.13.peg.243; -.
DR eggNOG; COG4257; Bacteria.
DR HOGENOM; CLU_054751_1_0_9; -.
DR Proteomes; UP000000606; Chromosome.
DR GO; GO:0016835; F:carbon-oxygen lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0017001; P:antibiotic catabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_01282; VirginiamycinB_lyase; 1.
DR InterPro; IPR011217; Streptogrm_lyase.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR PIRSF; PIRSF026412; Streptogrm_lyase; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Lyase; Magnesium; Metal-binding; Reference proteome.
FT CHAIN 1..295
FT /note="Virginiamycin B lyase"
FT /id="PRO_0000313767"
FT ACT_SITE 269
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01282"
FT BINDING 227
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01282"
FT BINDING 267
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01282"
FT BINDING 284
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01282"
SQ SEQUENCE 295 AA; 31344 MW; 5888A04C551867CB CRC64;
MQIRMTEYQV PDPESGPYGI TVCANGRIWF TEQKGNRIGM LTESGDITEY TIPTESAGAS
IITSGIDGEL WFTEYKAGKI GKITPQGKIT EYALPPGAAP FGIAAGCDDA MWYTDMAGHQ
IGRLSSSGEI TEYKLPKPGA FPSFITRGAD GALWFTQNQS GSIGRITADG DISEYPLPQE
QSGPVGITAG PDGALWFTEI NANQIGRITV SGKISEYQLP TAHARPHAIA AGGDGALWFT
EWGAGQIGRI TVDGDITEYP IPTADSEPHG IAAGSAHSIW FAEECGRIGK ISIQN
