ID   VGB_STRCO               Reviewed;         299 AA.
AC   Q9KZX7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Virginiamycin B lyase;
DE            EC=4.2.99.-;
DE   AltName: Full=Streptogramin B lyase;
GN   Name=vgb; OrderedLocusNames=SCO4449; ORFNames=SCD6.27c;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
RN   [2]
RP   FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=11467949; DOI=10.1021/bi0106787;
RA   Mukhtar T.A., Koteva K.P., Hughes D.W., Wright G.D.;
RT   "Vgb from Staphylococcus aureus inactivates streptogramin B antibiotics by
RT   an elimination mechanism not hydrolysis.";
RL   Biochemistry 40:8877-8886(2001).
CC   -!- FUNCTION: Inactivates the type B streptogramin antibiotics by
CC       linearizing the lactone ring at the ester linkage, generating a free
CC       phenylglycine carboxylate and converting the threonyl moiety into 2-
CC       amino-butenoic acid. {ECO:0000269|PubMed:11467949}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=27 uM for quinupristin {ECO:0000269|PubMed:11467949};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Vgb family. {ECO:0000305}.
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DR   EMBL; AL939120; CAB88456.1; -; Genomic_DNA.
DR   RefSeq; NP_628616.1; NC_003888.3.
DR   RefSeq; WP_011029662.1; NZ_VNID01000017.1.
DR   AlphaFoldDB; Q9KZX7; -.
DR   SMR; Q9KZX7; -.
DR   STRING; 100226.SCO4449; -.
DR   PRIDE; Q9KZX7; -.
DR   DNASU; 1099889; -.
DR   GeneID; 1099889; -.
DR   KEGG; sco:SCO4449; -.
DR   PATRIC; fig|100226.15.peg.4519; -.
DR   eggNOG; COG4257; Bacteria.
DR   HOGENOM; CLU_054751_1_0_11; -.
DR   InParanoid; Q9KZX7; -.
DR   OMA; ALWFTEW; -.
DR   PhylomeDB; Q9KZX7; -.
DR   SABIO-RK; Q9KZX7; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0016835; F:carbon-oxygen lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0017001; P:antibiotic catabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_01282; VirginiamycinB_lyase; 1.
DR   InterPro; IPR011217; Streptogrm_lyase.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   PIRSF; PIRSF026412; Streptogrm_lyase; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Lyase; Magnesium; Metal-binding; Reference proteome.
FT   CHAIN           1..299
FT                   /note="Virginiamycin B lyase"
FT                   /id="PRO_0000313778"
FT   ACT_SITE        274
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         232
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         272
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         288
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   299 AA;  31124 MW;  0ADB9DCB4FBC81AF CRC64;
     MNEINESYDT DSVREFTVSD ADAGPYALAE GPDGALWFTL VHRGAVARRD PDDGRVTVHP
     VGDGPTVIAP GPDGALWFTE YRAHRIGRIT PEGHYASFAP LTPEGGPFGI TAGPDGAMWF
     TLSSADRVGR VTMDGEVTEH PAPGAFPSAL TAGPDGALWC TLNQGNAIGR LTPDGHGTAY
     PLPTPGAAPV GIAAGPDGAL WFTEIGAGRI GRITVTGDLT EYPLSDPAAR PHAVTAGPNG
     ALWFTEWGSG RVGRITVDGR VTSYPLSRTD CEPHGIAVHD GALWCALETG SLARIQVPA