ID   VGH_BPAL3               Reviewed;         330 AA.
AC   P03650;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Minor spike protein;
DE   AltName: Full=H protein;
DE   AltName: Full=Pilot protein;
GN   Name=H;
OS   Escherichia phage alpha3 (Bacteriophage alpha-3).
OC   Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes;
OC   Petitvirales; Microviridae; Bullavirinae; Alphatrevirus.
OX   NCBI_TaxID=10849;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=1532908; DOI=10.1016/0167-4781(92)90440-b;
RA   Kodaira K., Nakano K., Okada S., Taketo A.;
RT   "Nucleotide sequence of the genome of the bacteriophage alpha 3:
RT   interrelationship of the genome structure and the gene products with those
RT   of the phages, phi X174, G4 and phi K.";
RL   Biochim. Biophys. Acta 1130:277-288(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=2988629; DOI=10.1016/0167-4781(85)90110-1;
RA   Kodaira K., Nakano K., Taketo A.;
RT   "Function and structure of microvirid phage alpha 3 genome. DNA sequence of
RT   H gene and properties of missense H mutant.";
RL   Biochim. Biophys. Acta 825:255-260(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE OF 1-70.
RX   PubMed=387790; DOI=10.1016/s0021-9258(19)86359-7;
RA   Sims J., Capon D., Dressler D.;
RT   "dnaG (primase)-dependent origins of DNA replication. Nucleotide sequences
RT   of the negative strand initiation sites of bacteriophages St-1, phi K, and
RT   alpha 3.";
RL   J. Biol. Chem. 254:12615-12628(1979).
CC   -!- FUNCTION: Minor spike component of the viral shell. Involved in the
CC       ejection of the phage DNA in the host and is injected with the DNA in
CC       the periplasmic space of the host. Involved in the determination of the
CC       phage host-range (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: The virion is composed of 60 copies each of the F, G, and J
CC       proteins, and 12 copies of the H protein. There are 12 spikes which are
CC       each composed of 5 G and one H proteins.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
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DR   EMBL; X60322; CAA42883.1; -; Genomic_DNA.
DR   EMBL; M25640; AAA32174.1; -; Genomic_DNA.
DR   EMBL; J02444; AAA32176.1; -; Genomic_RNA.
DR   PIR; A21537; ZHBPA3.
DR   RefSeq; NP_039599.1; NC_001330.1.
DR   SMR; P03650; -.
DR   GeneID; 1260699; -.
DR   KEGG; vg:1260699; -.
DR   Proteomes; UP000002137; Genome.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR006777; Microvir_H.
DR   Pfam; PF04687; Microvir_H; 1.
DR   PIRSF; PIRSF004160; Microvir_H; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Reference proteome;
KW   Viral genome ejection through host cell envelope;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   CHAIN           1..330
FT                   /note="Minor spike protein"
FT                   /id="PRO_0000164898"
FT   REGION          57..79
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   330 AA;  34844 MW;  B194EF44A83E3CE8 CRC64;
     MLGAVVGGIA SALASGAASK LFGGSQRGVS VMQQGAESAG LTNGGGAISM DNDQGIQSAI
     QGSNVPPAGQ LPESQNSGVM ADAGNMLRNA GKSLLDGTIQ AGSDKVKQAL IDKLIGGNDA
     KDRGKATRDY LAAAFPELNP WERAGAGAST AGLESSAQNQ QKEMLKMQLD NQKDIAKMQM
     DNNLQIAGIQ SATSRQNTKD SVYAQNEMLQ YNQRESQARV ASILANTDLT TKQATHEIMR
     MALTRAQETG QHLTNSQIMA LEKKVYAEIG KIHQDTQNSR YGSSQVTAAA KDVTNMITDA
     ASGASDWVSQ QWNSFFKDGK SNGISLNTRK