ID   VGH_BPPHK               Reviewed;         332 AA.
AC   P03649;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Minor spike protein;
DE   AltName: Full=H protein;
DE   AltName: Full=Pilot protein;
GN   Name=H;
OS   Enterobacteria phage phiK (Bacteriophage phi-K).
OC   Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes;
OC   Petitvirales; Microviridae; Bullavirinae; Alphatrevirus.
OX   NCBI_TaxID=10848;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (PHI-K AND MUTANT PHI KHT).
RX   PubMed=8827438; DOI=10.1093/oxfordjournals.jbchem.a021348;
RA   Kodaira K., Oki M., Kakikawa M., Kimoto H., Taketo A.;
RT   "The virion proteins encoded by bacteriophage phi K and its host-range
RT   mutant phi KhT: host-range determination and DNA binding properties.";
RL   J. Biochem. 119:1062-1069(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE OF 1-42.
RX   PubMed=387790; DOI=10.1016/s0021-9258(19)86359-7;
RA   Sims J., Capon D., Dressler D.;
RT   "dnaG (primase)-dependent origins of DNA replication. Nucleotide sequences
RT   of the negative strand initiation sites of bacteriophages St-1, phi K, and
RT   alpha 3.";
RL   J. Biol. Chem. 254:12615-12628(1979).
CC   -!- FUNCTION: Minor spike component of the viral shell. Involved in the
CC       ejection of the phage DNA in the host and is injected with the DNA in
CC       the periplasmic space of the host. Involved in the determination of the
CC       phage host-range.
CC   -!- SUBUNIT: The virion is composed of 60 copies each of the F, G, and J
CC       proteins, and 12 copies of the H protein. There are 12 spikes which are
CC       each composed of 5 G and one H proteins.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC   -!- MISCELLANEOUS: Phi KhT, a host-range mutant of phi K, can grow on
CC       E.coli C and B, besides K12, and is more thermosensitive than the
CC       parental phage phi K.
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DR   EMBL; X60323; CAA42893.1; -; Genomic_DNA.
DR   EMBL; M10726; AAA32365.1; -; Genomic_RNA.
DR   PIR; JC4807; JC4807.
DR   RefSeq; NP_043951.1; NC_001730.1.
DR   SMR; P03649; -.
DR   TCDB; 1.K.3.1.2; the phix174 tube-forming spike protein h (phix174-h) family.
DR   GeneID; 1261201; -.
DR   KEGG; vg:1261201; -.
DR   Proteomes; UP000002122; Genome.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR006777; Microvir_H.
DR   Pfam; PF04687; Microvir_H; 1.
DR   PIRSF; PIRSF004160; Microvir_H; 1.
PE   4: Predicted;
KW   Capsid protein; Viral genome ejection through host cell envelope;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   CHAIN           1..332
FT                   /note="Minor spike protein"
FT                   /id="PRO_0000164900"
FT   VARIANT         67
FT                   /note="V -> A (in phi KhT mutant)"
SQ   SEQUENCE   332 AA;  35110 MW;  C895595D1F9C5A7A CRC64;
     MLGSIIGGIG SSLLGGIASG GISSLLNKMF SKMPEHAASS AGLTNGQGTI GMDTDAGIQS
     AIQGSNVPPA GQLPASNTSG VMADAGNMIR NAGRALLDGT IQAGSDKVKE ALIDKLVGGN
     DAKDRGKATR DYLAAAFPEL NPWERAGAGA STAGLESSAQ NQQKEMLKMQ LDNQKDIAKM
     QMNNNLQIAG IQSATSRQNT KDSVYAQNEM LQYNQRESQA RVASILANTD LTTKQATHEI
     MRMALTRAQE TGQHLTNSQI MALEKKVYAE IGKIHQDTQN SRYGSSQVTA AAKDVTNMIT
     DASSGLADWA SQQWDSFFKD GKSDGIPLNT RK