VGR1A_PSEAE
ID VGR1A_PSEAE Reviewed; 643 AA.
AC Q9I741;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Type VI secretion system spike protein VgrG1a {ECO:0000303|PubMed:21325275};
DE AltName: Full=T6SS spike protein VgrG1a;
GN Name=vgrG1a {ECO:0000303|PubMed:21325275}; OrderedLocusNames=PA0091;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP SUBCELLULAR LOCATION, SUBUNIT, FUNCTION, INTERACTION WITH HCP1, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=21325275; DOI=10.1074/jbc.m110.193045;
RA Hachani A., Lossi N.S., Hamilton A., Jones C., Bleves S., Albesa-Jove D.,
RA Filloux A.;
RT "Type VI secretion system in Pseudomonas aeruginosa: secretion and
RT multimerization of VgrG proteins.";
RL J. Biol. Chem. 286:12317-12327(2011).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=PAK;
RX PubMed=24794869; DOI=10.1074/jbc.m114.563429;
RA Hachani A., Allsopp L.P., Oduko Y., Filloux A.;
RT "The VgrG proteins are 'a la carte' delivery systems for bacterial type VI
RT effectors.";
RL J. Biol. Chem. 289:17872-17884(2014).
RN [4]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=26894531; DOI=10.1107/s2059798315021142;
RA Spinola-Amilibia M., Davo-Siguero I., Ruiz F.M., Santillana E.,
RA Medrano F.J., Romero A.;
RT "The structure of VgrG1 from Pseudomonas aeruginosa, the needle tip of the
RT bacterial type VI secretion system.";
RL Acta Crystallogr. 72:22-33(2016).
RN [5] {ECO:0007744|PDB:6H3L, ECO:0007744|PDB:6H3N}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.25 ANGSTROMS), FUNCTION, AND
RP INTERACTION WITH TSE6 AND EAGT6.
RX PubMed=30177742; DOI=10.1038/s41564-018-0238-z;
RA Quentin D., Ahmad S., Shanthamoorthy P., Mougous J.D., Whitney J.C.,
RA Raunser S.;
RT "Mechanism of loading and translocation of type VI secretion system
RT effector Tse6.";
RL Nat. Microbiol. 3:1142-1152(2018).
CC -!- FUNCTION: Part of the H1 type VI secretion system (H1-T6SS) specialized
CC secretion system, which delivers several virulence factors in both
CC prokaryotic and eukaryotic cells during infection (PubMed:21325275,
CC PubMed:24794869). Forms the spike at the tip of the elongating tube
CC formed by haemolysin co-regulated protein 1/Hcp1 (PubMed:26894531).
CC Allows the delivery of the Tse6 toxin to target cells where it exerts
CC its toxicity (PubMed:30177742). {ECO:0000269|PubMed:21325275,
CC ECO:0000269|PubMed:24794869, ECO:0000269|PubMed:26894531,
CC ECO:0000269|PubMed:30177742}.
CC -!- SUBUNIT: Forms homotrimers (PubMed:21325275). Part of the type VI
CC secretion system (T6SS) (PubMed:26894531). Interacts with EagT6 and
CC Tse6; these interactions are required for Tse6 loading onto VgrG1
CC (PubMed:30177742). Interacts with Hcp1 (PubMed:21325275).
CC {ECO:0000269|PubMed:21325275, ECO:0000269|PubMed:26894531,
CC ECO:0000269|PubMed:30177742}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21325275}.
CC -!- DISRUPTION PHENOTYPE: Deletion of vgrG1a shows residual type VI
CC secretion (PubMed:21325275). However, simultaneous deletion of all
CC three vgrG genes (vgrG1a, vgrG1b and vgrG1c) totally abrogates
CC bacterial killing (PubMed:24794869). {ECO:0000269|PubMed:21325275,
CC ECO:0000269|PubMed:24794869}.
CC -!- SIMILARITY: Belongs to the VgrG protein family. {ECO:0000305}.
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DR EMBL; AE004091; AAG03481.1; -; Genomic_DNA.
DR PIR; H83635; H83635.
DR RefSeq; NP_248781.1; NC_002516.2.
DR RefSeq; WP_010895494.1; NZ_QZGE01000015.1.
DR PDB; 4MTK; X-ray; 3.32 A; A/B/C/D/E/F=1-643.
DR PDB; 4UHV; X-ray; 2.00 A; A/B=1-643.
DR PDB; 6H3L; EM; 4.20 A; A/B/C=1-643.
DR PDB; 6H3N; EM; 3.25 A; A/B/C=1-643.
DR PDBsum; 4MTK; -.
DR PDBsum; 4UHV; -.
DR PDBsum; 6H3L; -.
DR PDBsum; 6H3N; -.
DR AlphaFoldDB; Q9I741; -.
DR SMR; Q9I741; -.
DR STRING; 287.DR97_3048; -.
DR PaxDb; Q9I741; -.
DR PRIDE; Q9I741; -.
DR EnsemblBacteria; AAG03481; AAG03481; PA0091.
DR GeneID; 879511; -.
DR KEGG; pae:PA0091; -.
DR PATRIC; fig|208964.12.peg.95; -.
DR PseudoCAP; PA0091; -.
DR HOGENOM; CLU_004121_3_0_6; -.
DR InParanoid; Q9I741; -.
DR OMA; NIARPHA; -.
DR PhylomeDB; Q9I741; -.
DR BioCyc; PAER208964:G1FZ6-93-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0033104; C:type VI protein secretion system complex; IDA:PseudoCAP.
DR GO; GO:0033103; P:protein secretion by the type VI secretion system; IDA:PseudoCAP.
DR Gene3D; 2.40.50.230; -; 1.
DR InterPro; IPR006531; Gp5/Vgr_OB.
DR InterPro; IPR017847; T6SS_RhsGE_Vgr_subset.
DR InterPro; IPR006533; T6SS_Vgr_RhsGE.
DR InterPro; IPR037026; Vgr_OB-fold_dom_sf.
DR Pfam; PF04717; Phage_base_V; 1.
DR TIGRFAMs; TIGR01646; vgr_GE; 1.
DR TIGRFAMs; TIGR03361; VI_Rhs_Vgr; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Secreted.
FT CHAIN 1..643
FT /note="Type VI secretion system spike protein VgrG1a"
FT /id="PRO_0000448911"
FT STRAND 4..6
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 7..10
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 19..24
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 33..43
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 48..53
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 55..61
FT /evidence="ECO:0007829|PDB:6H3N"
FT HELIX 63..65
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 73..79
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 87..94
FT /evidence="ECO:0007829|PDB:4UHV"
FT HELIX 98..102
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 104..113
FT /evidence="ECO:0007829|PDB:6H3N"
FT HELIX 114..120
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 130..133
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 140..146
FT /evidence="ECO:0007829|PDB:4MTK"
FT STRAND 148..150
FT /evidence="ECO:0007829|PDB:4UHV"
FT HELIX 151..159
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 165..169
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 176..180
FT /evidence="ECO:0007829|PDB:4UHV"
FT HELIX 183..185
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 193..197
FT /evidence="ECO:0007829|PDB:6H3N"
FT TURN 200..202
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 206..208
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 210..218
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 223..225
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 240..243
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 247..250
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 254..256
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 263..265
FT /evidence="ECO:0007829|PDB:4UHV"
FT TURN 266..268
FT /evidence="ECO:0007829|PDB:4UHV"
FT HELIX 269..272
FT /evidence="ECO:0007829|PDB:4UHV"
FT TURN 273..275
FT /evidence="ECO:0007829|PDB:4UHV"
FT HELIX 277..280
FT /evidence="ECO:0007829|PDB:4UHV"
FT TURN 281..283
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 284..290
FT /evidence="ECO:0007829|PDB:4MTK"
FT STRAND 299..305
FT /evidence="ECO:0007829|PDB:6H3N"
FT HELIX 309..311
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 323..325
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 339..348
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 349..351
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 368..373
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 388..391
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 400..402
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 419..422
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 430..435
FT /evidence="ECO:0007829|PDB:4UHV"
FT HELIX 436..438
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 442..448
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 452..454
FT /evidence="ECO:0007829|PDB:4UHV"
FT TURN 460..462
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 463..465
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 467..470
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 484..488
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 495..499
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 512..518
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 520..526
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 528..534
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 536..542
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 544..550
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 565..572
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 575..582
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 585..588
FT /evidence="ECO:0007829|PDB:4UHV"
FT STRAND 600..606
FT /evidence="ECO:0007829|PDB:6H3N"
FT STRAND 608..613
FT /evidence="ECO:0007829|PDB:6H3N"
FT TURN 621..624
FT /evidence="ECO:0007829|PDB:6H3N"
FT HELIX 626..636
FT /evidence="ECO:0007829|PDB:4UHV"
SQ SEQUENCE 643 AA; 72013 MW; 59C4B4D416C61D9C CRC64;
MQLTRLVQVD CPLGPDVLLL QRMEGREELG RLFAYELHLV SENPNLPLEQ LLGKPMSLSL
ELPGGSRRFF HGIVARCSQV AGHGQFAGYQ ATLRPWPWLL TRTSDCRIFQ NQSVPEIIKQ
VFRNLGFSDF EDALTRPYRE WEYCVQYRET SFDFISRLME QEGIYYWFRH EQKRHILVLS
DAYGAHRSPG GYASVPYYPP TLGHRERDHF FDWQMAREVQ PGSLTLNDYD FQRPGARLEV
RSNIARPHAA ADYPLYDYPG EYVQSQDGEQ YARNRIEAIQ AQHERVRLRG VVRGIGAGHL
FRLSGYPRDD QNREYLVVGA EYRVVQELYE TGSGGAGSQF ESELDCIDAS QSFRLLPQTP
VPVVRGPQTA VVVGPKGEEI WTDQYGRVKV HFHWDRHDQS NENSSCWIRV SQAWAGKNWG
SMQIPRIGQE VIVSFLEGDP DRPIITGRVY NAEQTVPYEL PANATQSGMK SRSSKGGTPA
NFNEIRMEDK KGAEQLYIHA ERNQDNLVEN DASLSVGHDR NKSIGHDELA RIGNNRTRAV
KLNDTLLVGG AKSDSVTGTY LIEAGAQIRL VCGKSVVEFN ADGTINISGS AFNLYASGNG
NIDTGGRLDL NSGGASEVDA KGKGVQGTID GQVQAMFPPP AKG
