ID   VGR1B_PSEAE             Reviewed;         741 AA.
AC   Q9I737;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Type VI secretion system spike protein VgrG1b {ECO:0000303|PubMed:21325275};
GN   Name=vgrG1b {ECO:0000303|PubMed:21325275}; OrderedLocusNames=PA0095;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=21325275; DOI=10.1074/jbc.m110.193045;
RA   Hachani A., Lossi N.S., Hamilton A., Jones C., Bleves S., Albesa-Jove D.,
RA   Filloux A.;
RT   "Type VI secretion system in Pseudomonas aeruginosa: secretion and
RT   multimerization of VgrG proteins.";
RL   J. Biol. Chem. 286:12317-12327(2011).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PAK;
RX   PubMed=24794869; DOI=10.1074/jbc.m114.563429;
RA   Hachani A., Allsopp L.P., Oduko Y., Filloux A.;
RT   "The VgrG proteins are 'a la carte' delivery systems for bacterial type VI
RT   effectors.";
RL   J. Biol. Chem. 289:17872-17884(2014).
RN   [4]
RP   FUNCTION.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=30455305; DOI=10.1073/pnas.1814181115;
RA   Pissaridou P., Allsopp L.P., Wettstadt S., Howard S.A., Mavridou D.A.I.,
RA   Filloux A.;
RT   "The Pseudomonas aeruginosa T6SS-VgrG1b spike is topped by a PAAR protein
RT   eliciting DNA damage to bacterial competitors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 115:12519-12524(2018).
CC   -!- FUNCTION: Part of the H1 type VI secretion system (H1-T6SS) specialized
CC       secretion system, which delivers several virulence factors in both
CC       prokaryotic and eukaryotic cells during infection (PubMed:21325275,
CC       PubMed:24794869). Allows the delivery of the Tse7 toxin to target cells
CC       where it exerts toxicity through its nuclease domain (PubMed:24794869,
CC       PubMed:30455305). {ECO:0000269|PubMed:21325275,
CC       ECO:0000269|PubMed:24794869, ECO:0000269|PubMed:30455305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21325275}.
CC   -!- DISRUPTION PHENOTYPE: Deletion neither impacts secretion of components
CC       of the H1-T6SS nor requires H1-T6SS components for its own secretion
CC       (PubMed:21325275). However, simultaneous deletion of all three vgrG
CC       genes (vgrG1a, vgrG1b and vgrG1c) totally abrogates bacterial killing
CC       (PubMed:24794869). {ECO:0000269|PubMed:21325275,
CC       ECO:0000269|PubMed:24794869}.
CC   -!- SIMILARITY: Belongs to the VgrG protein family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG03485.1; -; Genomic_DNA.
DR   PIR; D83633; D83633.
DR   RefSeq; NP_248785.1; NC_002516.2.
DR   RefSeq; WP_003147059.1; NZ_QZGE01000015.1.
DR   AlphaFoldDB; Q9I737; -.
DR   SMR; Q9I737; -.
DR   STRING; 287.DR97_3052; -.
DR   PaxDb; Q9I737; -.
DR   PRIDE; Q9I737; -.
DR   EnsemblBacteria; AAG03485; AAG03485; PA0095.
DR   GeneID; 880726; -.
DR   KEGG; pae:PA0095; -.
DR   PATRIC; fig|208964.12.peg.99; -.
DR   PseudoCAP; PA0095; -.
DR   HOGENOM; CLU_004121_3_0_6; -.
DR   InParanoid; Q9I737; -.
DR   OMA; RPFAYEL; -.
DR   PhylomeDB; Q9I737; -.
DR   BioCyc; PAER208964:G1FZ6-97-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033104; C:type VI protein secretion system complex; IMP:PseudoCAP.
DR   GO; GO:0033103; P:protein secretion by the type VI secretion system; IMP:PseudoCAP.
DR   Gene3D; 2.40.50.230; -; 1.
DR   InterPro; IPR006531; Gp5/Vgr_OB.
DR   InterPro; IPR010609; Gp5_C.
DR   InterPro; IPR017847; T6SS_RhsGE_Vgr_subset.
DR   InterPro; IPR006533; T6SS_Vgr_RhsGE.
DR   InterPro; IPR037026; Vgr_OB-fold_dom_sf.
DR   Pfam; PF06715; Gp5_C; 2.
DR   Pfam; PF04717; Phage_base_V; 1.
DR   TIGRFAMs; TIGR01646; vgr_GE; 1.
DR   TIGRFAMs; TIGR03361; VI_Rhs_Vgr; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Secreted.
FT   CHAIN           1..741
FT                   /note="Type VI secretion system spike protein VgrG1b"
FT                   /id="PRO_0000448912"
FT   REGION          614..678
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        614..669
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   741 AA;  82751 MW;  2D5947A40D7AB951 CRC64;
     MALAQQTRLV RVDSPLGAEV LQLQRMEGRE ELGRPFAYEL ELISENPDLP LDGLLGKPAS
     LALELHDGSR RHFHGIVAAC SQGSGNGQFA SYQVTLRPWL WLLTRTSDCR IFQNQKVPDI
     IKQVFRDLGF SDFEDALSRS YREWEYCVQY RETSFDFVSR LMEQEGIYYW FRHEQSRHIL
     VLSDAYGAHQ SPPNYASVPY YPPTLEQRER DHFYDWHMAR EVQSGSLSLN DYDFQRPGAR
     LEVRSNIARS HAAADYPLYD YPGEYVQSQD GEQYARTRIE ALQARYERVR LRGRARGLGS
     GHLFKLSGYP REDQNREYLV VGAEYRVVQE LYETGGGGVG AQFESELDCI DAGQAYRPLP
     TTPLPIVRGP QTAVVVGPKG EEIWTDQYGR VKVHFHWDRH DQSNENSSCW IRVSQAWAGK
     NWGSMQIPRI GQEVIVSFLE GDPDRPIITG RVYNAEQTVP YELPANATQS GMKSRSSKGG
     TPANFNEIRM EDKKGAEQLF IHAEKNQDIE VENDETHWVG HDRTKTIDHD ETVHVKHDRT
     ETVDNNETIT IGVDRTEKVG NNEKISIGAN RTEDVGSNET ISIGVDRTEK VGSNEKISIG
     ANRTEDVGND ETISIGANRS ESVGNNETIS IGADRSESVG ANETIDIGGN QSTSIGKNES
     RSVGQGRDTS VGKDDSLDVG KSFTLNAGDS ITLVTGAASI RMKKDGSIVI SGKNITIDGS
     GAINVKADKN VVVKGRKILQ N