VGR1C_PSEAE
ID VGR1C_PSEAE Reviewed; 726 AA.
AC Q9I0F3;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Type VI secretion system spike protein VgrG1c {ECO:0000303|PubMed:21325275};
GN Name=vgrG1c {ECO:0000303|PubMed:21325275}; OrderedLocusNames=PA2685;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP SUBCELLULAR LOCATION, SUBUNIT, FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=21325275; DOI=10.1074/jbc.m110.193045;
RA Hachani A., Lossi N.S., Hamilton A., Jones C., Bleves S., Albesa-Jove D.,
RA Filloux A.;
RT "Type VI secretion system in Pseudomonas aeruginosa: secretion and
RT multimerization of VgrG proteins.";
RL J. Biol. Chem. 286:12317-12327(2011).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=PAK;
RX PubMed=24794869; DOI=10.1074/jbc.m114.563429;
RA Hachani A., Allsopp L.P., Oduko Y., Filloux A.;
RT "The VgrG proteins are 'a la carte' delivery systems for bacterial type VI
RT effectors.";
RL J. Biol. Chem. 289:17872-17884(2014).
CC -!- FUNCTION: Part of the H1 type VI secretion system (H1-T6SS) specialized
CC secretion system, which delivers several virulence factors in both
CC prokaryotic and eukaryotic cells during infection (PubMed:21325275,
CC PubMed:24794869). Allows the delivery of the Tse5/RhsP1 toxin to target
CC cells where it exerts its toxicity (PubMed:24794869).
CC {ECO:0000269|PubMed:21325275, ECO:0000269|PubMed:24794869}.
CC -!- SUBUNIT: Forms homomultimers (PubMed:21325275). Part of the type VI
CC secretion system (T6SS) (PubMed:21325275).
CC {ECO:0000269|PubMed:21325275}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21325275}.
CC -!- DISRUPTION PHENOTYPE: Deletion of vgrG1c shows residual type VI
CC secretion (PubMed:21325275). However, simultaneous deletion of all
CC three vgrG genes (vgrG1a, vgrG1b and vgrG1c) totally abrogates
CC bacterial killing (PubMed:24794869). {ECO:0000269|PubMed:21325275,
CC ECO:0000269|PubMed:24794869}.
CC -!- SIMILARITY: Belongs to the VgrG protein family. {ECO:0000305}.
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DR EMBL; AE004091; AAG06073.1; -; Genomic_DNA.
DR PIR; G83310; G83310.
DR RefSeq; NP_251375.1; NC_002516.2.
DR RefSeq; WP_003143801.1; NC_002516.2.
DR AlphaFoldDB; Q9I0F3; -.
DR SMR; Q9I0F3; -.
DR STRING; 287.DR97_5274; -.
DR PaxDb; Q9I0F3; -.
DR EnsemblBacteria; AAG06073; AAG06073; PA2685.
DR GeneID; 879130; -.
DR KEGG; pae:PA2685; -.
DR PATRIC; fig|208964.12.peg.2809; -.
DR PseudoCAP; PA2685; -.
DR HOGENOM; CLU_004121_3_0_6; -.
DR InParanoid; Q9I0F3; -.
DR OMA; TITVHAN; -.
DR PhylomeDB; Q9I0F3; -.
DR BioCyc; PAER208964:G1FZ6-2725-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0033104; C:type VI protein secretion system complex; IDA:PseudoCAP.
DR GO; GO:0033103; P:protein secretion by the type VI secretion system; IDA:PseudoCAP.
DR Gene3D; 2.40.50.230; -; 1.
DR InterPro; IPR006531; Gp5/Vgr_OB.
DR InterPro; IPR010609; Gp5_C.
DR InterPro; IPR017847; T6SS_RhsGE_Vgr_subset.
DR InterPro; IPR006533; T6SS_Vgr_RhsGE.
DR InterPro; IPR037026; Vgr_OB-fold_dom_sf.
DR Pfam; PF06715; Gp5_C; 1.
DR Pfam; PF04717; Phage_base_V; 1.
DR TIGRFAMs; TIGR01646; vgr_GE; 1.
DR TIGRFAMs; TIGR03361; VI_Rhs_Vgr; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Secreted.
FT CHAIN 1..726
FT /note="Type VI secretion system spike protein VgrG1c"
FT /id="PRO_0000448913"
FT REGION 502..522
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 502..520
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 726 AA; 81652 MW; 117FEFD6296340A7 CRC64;
MAIGQPFATA LGCTIIWRAA GALFGPPAAG DYQGRFPMLF SQHTRLVHVD SPLGPEVLQL
QRLEGREELG RLFSHELELV SSNPALPLDA LLGKPMSLAL ELPGGSRRYF HGIVARCSQG
AGAGQFASYQ VTLRPWLWLL TRTSDCRIFQ NQKVPDIIKQ VFRDLGFSDF EDALSRSYRE
WEYCVQYRET SFDFVSRLME QEGIYYWFRH EKKRHILVLS DAYGAHHSPA GYTSVPYYPP
SLGHRERDHF FDWHMAREVQ PGSLSLNDYD FQRPGTRLEV RSNVGRAHAA ADYPLYDYPG
EYVQSQDGEH YARTRIEAIQ TQYERVRLRG CARGIGAGHL FHLSNYPRLD QNREYLVVGA
EYRVVQELYE TGNGGGGAQF ESELDCIDAG QAFRPLPSTP VPVVRGPQTA VVVGPKGEEI
WTDQYGRVKV HFHWDRHDQS NENSSCWMRV SQAWAGKNWG SIQIPRIGQE VIVSFLEGDP
DRPIITGRVY NAEQTVPYEL PANATQSGTK SRSSKGGTPA NFNEIRMEDK KGAEQLFIHA
ERNQDIEVEN DESHWVGHDR TKTIDHDETV HVKHDRTETV DNNETITVHA NRSKTVDRNE
TVRIGMNKTE TILMASLQNV GMGRMENVGL GYSLNVGMMM NTVVGLNQST QVMKKKTLSV
GDSYEVSVGG SDDGSKITLD GQSITLGSQR IELTADREIL LRCGQSTIRL TPGEIEILSP
NVDINC
