ID   VGR2A_PSEAE             Reviewed;         842 AA.
AC   Q9I3K1;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Type VI secretion system spike protein VgrG2a {ECO:0000303|PubMed:26037124};
GN   Name=vgrG2a {ECO:0000303|PubMed:26037124}; OrderedLocusNames=PA1511;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=26037124; DOI=10.1128/mbio.00712-15;
RA   Sana T.G., Baumann C., Merdes A., Soscia C., Rattei T., Hachani A.,
RA   Jones C., Bennett K.L., Filloux A., Superti-Furga G., Voulhoux R.,
RA   Bleves S.;
RT   "Internalization of Pseudomonas aeruginosa Strain PAO1 into Epithelial
RT   Cells Is Promoted by Interaction of a T6SS Effector with the Microtubule
RT   Network.";
RL   MBio 6:E00712-E00712(2015).
CC   -!- FUNCTION: Part of the H2 type VI secretion system (H2-T6SS) specialized
CC       secretion system, which delivers several virulence factors in both
CC       prokaryotic and eukaryotic cells during infection (PubMed:26037124).
CC       May form the spike at the tip of the elongating tube formed by
CC       haemolysin co-regulated protein 2a/Hcp2a. In turn, may allow the
CC       delivery of the Tle4 antibacterial toxin to target cells where it
CC       exerts its toxicity (Probable). Promotes also the release of VgrG2b
CC       toxin to the host cell (PubMed:26037124). {ECO:0000269|PubMed:26037124,
CC       ECO:0000305|PubMed:26037124}.
CC   -!- DISRUPTION PHENOTYPE: Deletion leads to a 75% loss of internalization
CC       of P.aeruginosa into host cell. {ECO:0000269|PubMed:26037124}.
CC   -!- SIMILARITY: Belongs to the VgrG protein family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG04900.1; -; Genomic_DNA.
DR   PIR; C83458; C83458.
DR   RefSeq; NP_250202.1; NC_002516.2.
DR   RefSeq; WP_003147414.1; NZ_CP053028.1.
DR   AlphaFoldDB; Q9I3K1; -.
DR   SMR; Q9I3K1; -.
DR   IntAct; Q9I3K1; 1.
DR   MINT; Q9I3K1; -.
DR   STRING; 287.DR97_511; -.
DR   PaxDb; Q9I3K1; -.
DR   EnsemblBacteria; AAG04900; AAG04900; PA1511.
DR   GeneID; 883127; -.
DR   KEGG; pae:PA1511; -.
DR   PATRIC; fig|208964.12.peg.1563; -.
DR   PseudoCAP; PA1511; -.
DR   HOGENOM; CLU_004121_1_4_6; -.
DR   InParanoid; Q9I3K1; -.
DR   OMA; WDSTPFD; -.
DR   PhylomeDB; Q9I3K1; -.
DR   BioCyc; PAER208964:G1FZ6-1538-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0033104; C:type VI protein secretion system complex; IGC:PseudoCAP.
DR   GO; GO:0033103; P:protein secretion by the type VI secretion system; IGC:PseudoCAP.
DR   Gene3D; 2.40.50.230; -; 1.
DR   InterPro; IPR006531; Gp5/Vgr_OB.
DR   InterPro; IPR028244; T6SS_Rhs_Vgr_dom.
DR   InterPro; IPR017847; T6SS_RhsGE_Vgr_subset.
DR   InterPro; IPR006533; T6SS_Vgr_RhsGE.
DR   InterPro; IPR037026; Vgr_OB-fold_dom_sf.
DR   InterPro; IPR018769; VgrG2_C_DUF2345.
DR   Pfam; PF10106; DUF2345; 1.
DR   Pfam; PF04717; Phage_base_V; 1.
DR   Pfam; PF13296; T6SS_Vgr; 1.
DR   TIGRFAMs; TIGR01646; vgr_GE; 1.
DR   TIGRFAMs; TIGR03361; VI_Rhs_Vgr; 1.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..842
FT                   /note="Type VI secretion system spike protein VgrG2a"
FT                   /id="PRO_0000448914"
FT   REGION          265..291
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        265..289
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   842 AA;  93636 MW;  D10FFF81D8AEF356 CRC64;
     MRQRDLKFTF VVGEGKLAFD VVEFELEEAL CEPFRLNLKL ASDKNAIDFR QVLDQPGTFT
     LWQDGRPARY VHGIVSHFTQ GSSGFRRTRY ELLLEPQLAR LELCCNWRIF QEKSVPEILQ
     ALLKEHRVLD YEQRIYHEHL PREYCVQAGD SDHYLHDRLA FEEGLVYYFR FDEHRHTLVC
     SDRLYVQERI AGGPVLFSAQ PEGDNPQPVL HSFRYSENVR TARQTQRDYS FKRPTYDQEH
     HLAGEALEHQ GSSYERYDYP GRYKRSGAGR PFSESRLRGH RRDARVASVS GDDPRLIPGH
     AFALEGHPRA DFNAWWRPVR VVHRGTQYAG QEEESADAPL GVSYDLRAEL VPEDVEWRPA
     PLPRPRIDGP QIATVVGPAG EEIHCDEWGR VKVQFPWDRE GRHDEFSTCW IRVAQNWAGA
     DWGHMAIPRI GQEVIVDYLD GDCDQPIVTG RTYRATNRPP YALPDHKILS TIKSKEYKGS
     RANELRIDDT TAQISAALMS DHGASALHLG YLTHPRPEGG KPRGEGFELR TDEHGAVRAA
     KGLLLSTEEQ LRAGAGHLDR GVVVQVLEAA LKLARELGDY AGEHQGVGHD AAPQQTLQEA
     VRDLGHGAND ESGKSNGGKP AIALSGPAGI AAATPASLTL AAGEHVDSVA RQNQQVTAGQ
     KVVINAGSDI GLFAQGGELR QITHQGPMLL QAQKNDIRLE AEQSVEVSAS QQHVLVTAKE
     HITLMCGGAY LTLKGGNIEL GMPGNFVVKA AKHSHVGPAH ASTSFNAWDS TPFDDRYVLR
     DEATLEPLPN IAVEVIRGDG GVVKLMTDSQ GRLPKQQHLA MDPVQIRILG KGSHNSDTES
     NT