ID   VGR4B_PSEAE             Reviewed;         808 AA.
AC   Q9HYC3;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Type VI secretion system spike protein VgrG4b {ECO:0000303|PubMed:31417515};
GN   Name=vgrG4b {ECO:0000303|PubMed:31417515}; OrderedLocusNames=PA3486;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=31417515; DOI=10.3389/fmicb.2019.01718;
RA   Wettstadt S., Wood T.E., Fecht S., Filloux A.;
RT   "Delivery of the Pseudomonas aeruginosa Phospholipase Effectors PldA and
RT   PldB in a VgrG- and H2-T6SS-Dependent Manner.";
RL   Front. Microbiol. 10:1718-1718(2019).
CC   -!- FUNCTION: Part of the H2 type VI secretion system (H2-T6SS) specialized
CC       secretion system, which delivers several virulence factors in both
CC       prokaryotic and eukaryotic cells during infection. Allows the delivery
CC       of the phospholipase effector PldA to target cells where it exerts its
CC       toxicity. {ECO:0000269|PubMed:31417515}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31417515}.
CC   -!- DISRUPTION PHENOTYPE: Deletion leads to a complete loss of PldA
CC       secretion. {ECO:0000269|PubMed:31417515}.
CC   -!- SIMILARITY: Belongs to the VgrG protein family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG06874.1; -; Genomic_DNA.
DR   PIR; G83210; G83210.
DR   RefSeq; NP_252176.1; NC_002516.2.
DR   RefSeq; WP_003147991.1; NZ_CP053028.1.
DR   AlphaFoldDB; Q9HYC3; -.
DR   SMR; Q9HYC3; -.
DR   STRING; 287.DR97_4636; -.
DR   PaxDb; Q9HYC3; -.
DR   EnsemblBacteria; AAG06874; AAG06874; PA3486.
DR   GeneID; 879939; -.
DR   KEGG; pae:PA3486; -.
DR   PATRIC; fig|208964.12.peg.3650; -.
DR   PseudoCAP; PA3486; -.
DR   HOGENOM; CLU_004121_7_0_6; -.
DR   InParanoid; Q9HYC3; -.
DR   OMA; HSHRTIG; -.
DR   PhylomeDB; Q9HYC3; -.
DR   BioCyc; PAER208964:G1FZ6-3554-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033104; C:type VI protein secretion system complex; IGC:PseudoCAP.
DR   GO; GO:0033103; P:protein secretion by the type VI secretion system; IGC:PseudoCAP.
DR   Gene3D; 2.40.50.230; -; 1.
DR   InterPro; IPR006531; Gp5/Vgr_OB.
DR   InterPro; IPR017847; T6SS_RhsGE_Vgr_subset.
DR   InterPro; IPR006533; T6SS_Vgr_RhsGE.
DR   InterPro; IPR037026; Vgr_OB-fold_dom_sf.
DR   Pfam; PF04717; Phage_base_V; 1.
DR   TIGRFAMs; TIGR01646; vgr_GE; 1.
DR   TIGRFAMs; TIGR03361; VI_Rhs_Vgr; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Secreted.
FT   CHAIN           1..808
FT                   /note="Type VI secretion system spike protein VgrG4b"
FT                   /id="PRO_0000448916"
SQ   SEQUENCE   808 AA;  90635 MW;  0B6B7851333C1056 CRC64;
     MFNPANQTHF SLSLDGLRHD LQVLEFSGHE GISRPYRFEL ELVSERAGLD LEALMHRPAF
     LAFTPQGQGV HGLVYGAAQG DAGKRLTRYR LTLVPHLAYL AQRNNQRIFQ HLTVPQIVAL
     ILEEHGILAD AYRFQLGTRY PEREYCVQYD ESDLHFVQRL CAEEGIHFHF RHSAEAHLLV
     FGDDQTVFPR LGRPTAYVHD SGLVADEPVI KRFSLRLASR TTRTTRRDYD FEKPRLLLEA
     GNRPAADAPA EPDLEDYDYP GRFVDRQRGK LLSQRALERH RADRRLGEGV SDQPLLVSGH
     FLEIAEHPRA EWNDLWLLSE VFHEGKQPQV LEENVTSDTS ASTDDFQQGY RNRFLATPWE
     VFFRPPLEHP KPRVLGSQTA VVTGPPGEEI HCDRYGRVRV QFHWDREGQG DDKSSCWLRV
     ASGWAGNGYG GIVIPRVGME VLVDFLEGDP DQPLVSGCVY HAAHPVPYEL PANQTRSVFK
     SLSSPGGDGY NELRIEDRKG QEQIFVHAQR DWDENIEHDQ KIRVGHERHD TVEANSYSEF
     KAEEHHTVHG ERKVELKADD HLTVGDSQHV KLGRAYLARA GREIHLKAGQ KMVIEADSEL
     TVKAGGSFIR LDASGIAISG PLARINAGGA PGSGSGIAIK MPLTPGAADA DVAGRPLQPA
     NAGLHASDPK QNGEYRFDIR LQDIPGDEGF PLIHTPWRIV QGKEHNLVLE GESDEKGRLV
     LDDTQQRQLS NACERAPGDV WLVYPGQRIG IRPHREREGW DATRHALGAL DFHDTLGGQR
     APTPLEHQRG KLDSCCEGDL YSHLLAKD