ID   VGRG1_AERHY             Reviewed;         681 AA.
AC   K7WKL8;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2013, sequence version 1.
DT   03-AUG-2022, entry version 19.
DE   RecName: Full=Type VI secretion system spike protein VgrG1 {ECO:0000303|PubMed:19880608};
DE   AltName: Full=Actin ADP-ribosyltransferase {ECO:0000303|PubMed:19880608};
DE            EC=2.4.2.31 {ECO:0000269|PubMed:19880608};
GN   Name=vgrG1;
OS   Aeromonas hydrophila.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=644;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SSU;
RX   PubMed=23519162; DOI=10.1099/mic.0.063495-0;
RA   Sha J., Rosenzweig J.A., Kozlova E.V., Wang S., Erova T.E., Kirtley M.L.,
RA   van Lier C.J., Chopra A.K.;
RT   "Evaluation of the roles played by Hcp and VgrG type 6 secretion system
RT   effectors in Aeromonas hydrophila SSU pathogenesis.";
RL   Microbiology 159:1120-1135(2013).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, AND CATALYTIC ACTIVITY.
RX   PubMed=19880608; DOI=10.1128/jb.01260-09;
RA   Suarez G., Sierra J.C., Erova T.E., Sha J., Horneman A.J., Chopra A.K.;
RT   "A type VI secretion system effector protein, VgrG1, from Aeromonas
RT   hydrophila that induces host cell toxicity by ADP ribosylation of actin.";
RL   J. Bacteriol. 192:155-168(2010).
CC   -!- FUNCTION: Part of the type VI secretion system specialized secretion
CC       system, which delivers several virulence factors in both prokaryotic
CC       and eukaryotic cells during infection. Acts directly as an secreted
CC       effector with an actin ADP-ribosyltransferase activity that disrupts
CC       the host actin cytoskeleton, leading to a decrease in host cell
CC       viability and an increase in apoptosis. {ECO:0000269|PubMed:19880608}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-arginyl-[protein] + NAD(+) = H(+) + N(omega)-(ADP-D-
CC         ribosyl)-L-arginyl-[protein] + nicotinamide; Xref=Rhea:RHEA:19149,
CC         Rhea:RHEA-COMP:10532, Rhea:RHEA-COMP:15087, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17154, ChEBI:CHEBI:29965, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:142554; EC=2.4.2.31;
CC         Evidence={ECO:0000269|PubMed:19880608};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19880608}.
CC   -!- SIMILARITY: Belongs to the VgrG protein family. {ECO:0000305}.
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DR   EMBL; JX646703; AFX59895.1; -; Genomic_DNA.
DR   AlphaFoldDB; K7WKL8; -.
DR   SMR; K7WKL8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0106274; F:NAD+-protein-arginine ADP-ribosyltransferase activity; IEA:UniProtKB-EC.
DR   Gene3D; 2.40.50.230; -; 1.
DR   InterPro; IPR006531; Gp5/Vgr_OB.
DR   InterPro; IPR017847; T6SS_RhsGE_Vgr_subset.
DR   InterPro; IPR006533; T6SS_Vgr_RhsGE.
DR   InterPro; IPR037026; Vgr_OB-fold_dom_sf.
DR   Pfam; PF04717; Phage_base_V; 1.
DR   TIGRFAMs; TIGR01646; vgr_GE; 1.
DR   TIGRFAMs; TIGR03361; VI_Rhs_Vgr; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Nucleotidyltransferase; Secreted; Transferase.
FT   CHAIN           1..681
FT                   /note="Type VI secretion system spike protein VgrG1"
FT                   /id="PRO_0000448968"
FT   REGION          621..640
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   681 AA;  75614 MW;  6DA37BCA1F88451F CRC64;
     MADSTGLQFT VKVGALPENT FVVAEFALDE ALNRPFNLRL ELASAQPDID FGAVLDQPCE
     LLVWYNGELQ RRVCGVVSDF AQGDSGFRRT RYQLRVLPAL WRLSLRQNSR IFQAQKPDEI
     LSILLQEHGI TDYAFALKNE HAKREYCVQY RESDLDFVNR LAAEEGMFYF HEFEAGKHRI
     VFADDAAALT QGPELFFNLG NRSLEQGPYV RQFHYREAVR PSDVELKDYS FKTPAYGLSH
     KKVGAELTHQ RDTYQHFDFP GRYKEDPSGK AFAQHRLDAL RNDAVAGQAK SNCAALLPGQ
     SFSLTEHPNG SLNTDWQIVR IQHTGLQPQA LEEEGGSGPT VYHNEFGVVK ASTTWRARIG
     SPEAPHKPMV DGPQIAIVVG PDGEEIYCDE HGRVKLQFPW DRYGSSNDQS SCWVRVSQGW
     AGGQYGMMAI PRIGHEVIVS FLEGDPDQPI VTGRTYHATN RPPYELPANK TRTVLRTETH
     QGEGFNELRF EDQVGQEEIY IHGQKDLNVL IENDAAWHIK HDEHTDVDNE RVTRIKANDH
     LTVEGEKRDQ IKADYSLTVD TSMHQKLGDS WLTQAGQEVH VKAGAKVVLE AGSEITVKVG
     GCFIKVDGGG VTLVGPTIKM NSGGSPSSGS GWGGKSPVDP LGVSVPPKPK VPLTPAQLAT
     MKSAAPFCEE CEKCKEGGCE I