ID   VGRG2_VIBCH             Reviewed;         694 AA.
AC   Q9KNE7;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Type VI secretion system spike protein VgrG2;
GN   Name=vgrG2; OrderedLocusNames=VC_A0018;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=19131969; DOI=10.1038/emboj.2008.269;
RA   Boenemann G., Pietrosiuk A., Diemand A., Zentgraf H., Mogk A.;
RT   "Remodelling of VipA/VipB tubules by ClpV-mediated threading is crucial for
RT   type VI protein secretion.";
RL   EMBO J. 28:315-325(2009).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=21555399; DOI=10.1128/iai.01266-10;
RA   Miyata S.T., Kitaoka M., Brooks T.M., McAuley S.B., Pukatzki S.;
RT   "Vibrio cholerae requires the type VI secretion system virulence factor
RT   VasX to kill Dictyostelium discoideum.";
RL   Infect. Immun. 79:2941-2949(2011).
CC   -!- FUNCTION: Part of the type VI secretion system specialized secretion
CC       system, which delivers several virulence factors in both prokaryotic
CC       and eukaryotic cells during infection (PubMed:21555399). Forms the
CC       spike at the tip of the elongating tube formed by haemolysin co-
CC       regulated protein Hcp. Allows the delivery of the VasX antibacterial
CC       toxin to target cells where it exerts its toxicity (PubMed:21555399).
CC       {ECO:0000269|PubMed:21555399}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19131969}.
CC   -!- DISRUPTION PHENOTYPE: Mutants are unable to secrete VasX.
CC       {ECO:0000269|PubMed:21555399}.
CC   -!- SIMILARITY: Belongs to the VgrG protein family. {ECO:0000305}.
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DR   EMBL; AE003853; AAF95932.1; -; Genomic_DNA.
DR   PIR; F82511; F82511.
DR   RefSeq; NP_232419.1; NC_002506.1.
DR   RefSeq; WP_000212125.1; NZ_LT906615.1.
DR   AlphaFoldDB; Q9KNE7; -.
DR   SMR; Q9KNE7; -.
DR   STRING; 243277.VC_A0018; -.
DR   PRIDE; Q9KNE7; -.
DR   DNASU; 2612507; -.
DR   EnsemblBacteria; AAF95932; AAF95932; VC_A0018.
DR   GeneID; 57741493; -.
DR   KEGG; vch:VC_A0018; -.
DR   PATRIC; fig|243277.26.peg.2665; -.
DR   eggNOG; COG3501; Bacteria.
DR   HOGENOM; CLU_004121_7_2_6; -.
DR   OMA; HSHRTIG; -.
DR   BioCyc; VCHO:VCA0018-MON; -.
DR   Proteomes; UP000000584; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.40.50.230; -; 1.
DR   InterPro; IPR006531; Gp5/Vgr_OB.
DR   InterPro; IPR017847; T6SS_RhsGE_Vgr_subset.
DR   InterPro; IPR006533; T6SS_Vgr_RhsGE.
DR   InterPro; IPR037026; Vgr_OB-fold_dom_sf.
DR   Pfam; PF04717; Phage_base_V; 1.
DR   TIGRFAMs; TIGR01646; vgr_GE; 1.
DR   TIGRFAMs; TIGR03361; VI_Rhs_Vgr; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Secreted.
FT   CHAIN           1..694
FT                   /note="Type VI secretion system spike protein VgrG2"
FT                   /id="PRO_0000448966"
SQ   SEQUENCE   694 AA;  77212 MW;  1ABA36735F19CB33 CRC64;
     MATLAYSIEV EGLEDETLVV RGFHGQESLS NSVFLGQACY GFRYQMQLAS RVSNLTADQM
     VDKRAELKLY RNSQLVQRVH GIVRAFSQGD IGHHHTFYQL TLVPALERLS LRHNSRIFQK
     QTVPEILSIL LQEMGIHDYA FALKRDCVQR EFCVQYRESD IDFLHRLAAE EGLVYSFVHE
     AGKHTLYFSD ASDSLSKLPE PIPYNALAGG TMDTPYIHGL TYRTQAEVSE VQLKDYSFKK
     PAYSFLQTVQ GTELDYQQTR YQHFDAPGRY KDDVNGAAFS QIRLDYLRRH AHTATGQSNE
     PLLRAGYKFD LQEHLDPAMN RDWVVVSINH QGEQPQALQE EGGSGATTYN NQFSLIPGHL
     HWRAEPQPKP QVDGPMIATV VGPEGEEIFC DEHGRVKIHF PWDRYSNGNE QSSCWVRVSQ
     GWAGSQYGFI AIPRIGHEVI VSFLNGDPDQ PIITGRTYHA TNTPPYTLPE HKTKTVLRTE
     THQGEGFNEL SFEDQAGKEQ IYLHAQKDFD GLIENDHTTV IRHDQHLTVE NDQFTQIKHN
     QHLTVEGESR TLVKLDCSSE IGGSLQQKIG SKAIYDAGTE VHLKAGNKLV LEAGNELTIK
     AGGSFIKVDA GGVHVVGSAI NLNSGGSAGS GSGYGGKMAE LPQGVDKAKT PQEIELAAVT
     PTQQSMSPLL KARQIEALKG PAPVCEVCEE AKGN