ID   VGRG5_PSEAE             Reviewed;         790 AA.
AC   Q9HU93;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Type VI secretion system spike protein VgrG5 {ECO:0000303|PubMed:31417515};
GN   Name=vgrG5 {ECO:0000303|PubMed:31417515}; OrderedLocusNames=PA5090;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=31417515; DOI=10.3389/fmicb.2019.01718;
RA   Wettstadt S., Wood T.E., Fecht S., Filloux A.;
RT   "Delivery of the Pseudomonas aeruginosa Phospholipase Effectors PldA and
RT   PldB in a VgrG- and H2-T6SS-Dependent Manner.";
RL   Front. Microbiol. 10:1718-1718(2019).
CC   -!- FUNCTION: Part of the H2 type VI secretion system (H2-T6SS) specialized
CC       secretion system, which delivers several virulence factors in both
CC       prokaryotic and eukaryotic cells during infection. Allows the delivery
CC       of the phospholipase effector PldB to target cells where it exerts its
CC       toxicity. Also plays a role in VgrG4b and its effector PldA secretion.
CC       {ECO:0000269|PubMed:31417515}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31417515}.
CC   -!- DISRUPTION PHENOTYPE: Deletion leads to a complete loss of PldA, PldB
CC       and VgrG4b secretion. {ECO:0000269|PubMed:31417515}.
CC   -!- SIMILARITY: Belongs to the VgrG protein family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG08475.1; -; Genomic_DNA.
DR   PIR; D83011; D83011.
DR   RefSeq; NP_253777.1; NC_002516.2.
DR   RefSeq; WP_003114455.1; NZ_QZGE01000002.1.
DR   AlphaFoldDB; Q9HU93; -.
DR   SMR; Q9HU93; -.
DR   STRING; 287.DR97_2444; -.
DR   PaxDb; Q9HU93; -.
DR   PRIDE; Q9HU93; -.
DR   EnsemblBacteria; AAG08475; AAG08475; PA5090.
DR   GeneID; 882228; -.
DR   KEGG; pae:PA5090; -.
DR   PATRIC; fig|208964.12.peg.5335; -.
DR   PseudoCAP; PA5090; -.
DR   HOGENOM; CLU_004121_7_0_6; -.
DR   InParanoid; Q9HU93; -.
DR   OMA; GNDETMQ; -.
DR   PhylomeDB; Q9HU93; -.
DR   BioCyc; PAER208964:G1FZ6-5205-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033104; C:type VI protein secretion system complex; IGC:PseudoCAP.
DR   GO; GO:0033103; P:protein secretion by the type VI secretion system; IGC:PseudoCAP.
DR   Gene3D; 2.40.50.230; -; 1.
DR   InterPro; IPR006531; Gp5/Vgr_OB.
DR   InterPro; IPR017847; T6SS_RhsGE_Vgr_subset.
DR   InterPro; IPR006533; T6SS_Vgr_RhsGE.
DR   InterPro; IPR037026; Vgr_OB-fold_dom_sf.
DR   Pfam; PF04717; Phage_base_V; 1.
DR   TIGRFAMs; TIGR01646; vgr_GE; 1.
DR   TIGRFAMs; TIGR03361; VI_Rhs_Vgr; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Secreted.
FT   CHAIN           1..790
FT                   /note="Type VI secretion system spike protein VgrG5"
FT                   /id="PRO_0000448917"
FT   REGION          753..790
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   790 AA;  87822 MW;  14BB37157F4DCBA4 CRC64;
     MFAPANQTHF SLRIENLRHD FQVLAFRGRE AISQPFRFDL ELVSERSDLD LDALLHQPAF
     LILSPSGQGV HGQLTSIAQG DSGKRLTGYR ATLEPRLAYL GLCSDQRIFQ RLSVPEIIGR
     VLEGHGILAD AYRFQLGPTP YPAREYCTQY DETDLAFLSR LCEEEGIHYH HEFSAQGHVL
     VFGDDQTSFP RLQRPVAYVQ DAGLVADQPV VKRFGVRFDT RASRVTRRDY DFEQPALQMQ
     AAHGPQPGAQ QPDLEDYDYP GRFTHRERGK HLSHRALERH RADYLQARGE SDEPALLSGH
     FLTLSAHPRG EWNDLWLLTE VLHEGRQPQV LEESIDSDVA QGQGDFRQGY RNHFVATPWS
     AHFRPPLEHP RPRVLGCQTA VVTGPAGETI HCDQYGRVKV QFFWDRLGQA DDNTSCWLRV
     ASNWGGKRYG GVAIPRVGME VLVGFLEGDP DQPLVTGCLY HSENRVPYEL PQNKTRSVFK
     TDSYPGGGGF NELRIEDRKG QEQIFVHAQR DWDENIEHDQ KIRVGHERHD TVEGDSYSEF
     RAEEQRTVHA DRKVELKAAD HLSVADALHL RIGTGQFVEA GDEIHFKAGD KVVIEAGMEL
     TLKGGGSFAR LDPGGVTLDG AQVMINSGGS PGIGSGVRAL SPLQPLAADA AAAGGALLGA
     IAQKIGEAPQ KLLRFELSPL PGVASAARQP YRLYANGAFK EEGIADEGGA ISFEPLPGER
     TYRIETANGH AYEVEMVDQP DALQADDRLA QQGFRDYRAE MPQHKPRSAP DAYRRDASRP
     GAADKDEPTP