VHT1_YEAST
ID VHT1_YEAST Reviewed; 593 AA.
AC P53241; D6VUJ9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Vitamin H transporter;
DE AltName: Full=H(+)/biotin symporter;
GN Name=VHT1; OrderedLocusNames=YGR065C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP CHARACTERIZATION.
RX PubMed=10373489; DOI=10.1074/jbc.274.26.18741;
RA Stolz J., Hoja U., Meier S., Sauer N., Schweizer E.;
RT "Identification of the plasma membrane H+-biotin symporter of Saccharomyces
RT cerevisiae by rescue of a fatty acid-auxotrophic mutant.";
RL J. Biol. Chem. 274:18741-18746(1999).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; SER-33 AND SER-43, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Involved in uptake of biotin with the concomitant entry of
CC protons.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Allantoate
CC permease family. {ECO:0000305}.
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DR EMBL; Z72850; CAA97067.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08160.1; -; Genomic_DNA.
DR PIR; S64360; S64360.
DR RefSeq; NP_011579.1; NM_001181194.1.
DR AlphaFoldDB; P53241; -.
DR BioGRID; 33309; 230.
DR DIP; DIP-5519N; -.
DR IntAct; P53241; 10.
DR MINT; P53241; -.
DR STRING; 4932.YGR065C; -.
DR TCDB; 2.A.1.14.12; the major facilitator superfamily (mfs).
DR iPTMnet; P53241; -.
DR MaxQB; P53241; -.
DR PaxDb; P53241; -.
DR PRIDE; P53241; -.
DR EnsemblFungi; YGR065C_mRNA; YGR065C; YGR065C.
DR GeneID; 852956; -.
DR KEGG; sce:YGR065C; -.
DR SGD; S000003297; VHT1.
DR VEuPathDB; FungiDB:YGR065C; -.
DR eggNOG; KOG2533; Eukaryota.
DR HOGENOM; CLU_001265_4_2_1; -.
DR InParanoid; P53241; -.
DR OMA; WYAPDEI; -.
DR BioCyc; YEAST:G3O-30779-MON; -.
DR PRO; PR:P53241; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53241; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031224; C:intrinsic component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0015225; F:biotin transmembrane transporter activity; IDA:SGD.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015878; P:biotin transport; IDA:SGD.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Biotin; Cell membrane; Membrane; Phosphoprotein; Reference proteome;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..593
FT /note="Vitamin H transporter"
FT /id="PRO_0000121372"
FT TOPO_DOM 1..121
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 143..166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..190
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..224
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..291
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 313..361
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 383..408
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 430..432
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 454..460
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..481
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 482..492
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 514..526
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 527..547
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 548..593
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 32
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 33
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 43
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 593 AA; 69083 MW; 8B91D6247867386D CRC64;
MTISNKSWRS YFPHLRKLPE DDQYLYSDDT NSSIIAEEEL HHSVDKSSKT DVTAETTAVE
PHPHNLRHDL PYEVRDEAGR KWWKYFDEFE YRVNKEYKKS RKWYEFLYPN HTTQTKAERR
LLYKLDIIIA LYFFMLCWSK SVDLNNYTNA YVSNMKEDLN MKGNDYVYTS TIANVGAIVF
QLPFMYLLPR FPSHIILPVM DLGWTWFTFA CYRANSLAEL RAYRFILSAF GAAYYPVSQY
ILGCWYAPDE INSRVCLFFC GQQLGSVTSG LLQSRIFKSL NGVHGLAGWR WMFLIDAIAI
SLPTAIIGFF VIPGVPSKCY SLFLTDEEIR IARARNKRNQ IKDGVDKSKL APLWSRKLWK
KVFCTPAFWV LVVFDTCSWN NMTAYSGSYT LWLKSNTKYS IAQVNNLSVI PACLGFAYVI
FCAFGADLFR CKWIFMVFAA IMNTVSCALL IKWDIPSKAK WYAFFTTYFS VAASPCLWSF
INDFLRFDPQ VKAITWIAIY SFSQSTYAWI PTLAWPTVES PRFKTGYTVS LIFGAIYGLW
TFVVLFFYKR NEKKHALGNG IILYDSNKGE ELPEFVKKNM EERDGYYYLK RSS
