ID   VHUA_METJA              Reviewed;         418 AA.
AC   Q58592;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=F420-non-reducing hydrogenase vhu subunit A;
DE            EC=1.12.99.-;
GN   Name=vhuA; OrderedLocusNames=MJ1192;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000305};
CC   -!- SUBUNIT: The F420-non-reducing hydrogenase vhu is composed of four
CC       subunits; VhuA, VhuD, VhuG and VhuU. {ECO:0000250}.
CC   -!- MISCELLANEOUS: The large subunit of Vhu is split into VhuA and VhuU.
CC       Each contributes two ligands to the [NiFeSe] center.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC       family. {ECO:0000305}.
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DR   EMBL; L77117; AAB99194.1; -; Genomic_DNA.
DR   PIR; G64448; G64448.
DR   RefSeq; WP_010870705.1; NC_000909.1.
DR   AlphaFoldDB; Q58592; -.
DR   SMR; Q58592; -.
DR   STRING; 243232.MJ_1192; -.
DR   EnsemblBacteria; AAB99194; AAB99194; MJ_1192.
DR   GeneID; 1452611; -.
DR   KEGG; mja:MJ_1192; -.
DR   eggNOG; arCOG01549; Archaea.
DR   HOGENOM; CLU_044556_0_0_2; -.
DR   InParanoid; Q58592; -.
DR   OMA; QNNLAMN; -.
DR   OrthoDB; 32760at2157; -.
DR   PhylomeDB; Q58592; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   Gene3D; 1.10.645.10; -; 1.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   Pfam; PF00374; NiFeSe_Hases; 2.
DR   SUPFAM; SSF56762; SSF56762; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Oxidoreductase; Reference proteome.
FT   CHAIN           1..418
FT                   /note="F420-non-reducing hydrogenase vhu subunit A"
FT                   /id="PRO_0000199730"
FT   BINDING         61
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   BINDING         64
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   418 AA;  46652 MW;  0BF7333C53360FA4 CRC64;
     MGKIVIEPLS RLEGHGKVTI TLDENGKPKD VKLHITALRG FEQFVVGRPA EEVPRIVPRI
     CGICQTAHHL ASVKAIDAAW GVEIPEPAKK LRELMHIGNM IHSHALHFYF LAAPDFVLGP
     DADPAIRNIV GVIDKAPDVA KQAIALRKFG QKIVEAVGGK AIHPVTGIPG GQAKRLTEEE
     RDELLKDADQ MIEYAKNGVE LIKQLNEQYM EQIKTLGVID TYYLGLVKDG KHNFYDDTLR
     FLSPDGKEKV EFKPEEYLNY IGEYVVPYNY VKHPYYKKVG YPEGVYRVGP LAMLNVCDEM
     ETPLAEEYRK EFLEIFGFPA NQSLAYNHAR LIELVEACEK AKILLEDNDI TSDDIKADVE
     PKAGNGVGVV YAPRGVLIHN YETDENGIVV KANMIVATTH NVPTMEKAIQ QAAQVIFK