ID   VHUA_METVO              Reviewed;         420 AA.
AC   Q00407;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 2.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=F420-non-reducing hydrogenase vhu subunit A;
DE            EC=1.12.99.-;
GN   Name=vhuA;
OS   Methanococcus voltae.
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanococcaceae; Methanococcus.
OX   NCBI_TaxID=2188;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33273 / DSM 1537 / NBRC 100457 / OCM 70 / PS;
RX   PubMed=1603063; DOI=10.1007/bf00587582;
RA   Halboth S., Klein A.;
RT   "Methanococcus voltae harbors four gene clusters potentially encoding two
RT   [NiFe] and two [NiFeSe] hydrogenases, each of the cofactor F420-reducing or
RT   F420-non-reducing types.";
RL   Mol. Gen. Genet. 233:217-224(1992).
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000305};
CC   -!- SUBUNIT: The F420-non-reducing hydrogenase vhu is composed of four
CC       subunits; VhuA, VhuD, VhuG and VhuU. {ECO:0000250}.
CC   -!- MISCELLANEOUS: The large subunit of Vhu is split into VhuA and VhuU.
CC       Each contributes two ligands to the [NiFeSe] center.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC       family. {ECO:0000305}.
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DR   EMBL; X61204; CAA43510.1; -; Genomic_DNA.
DR   PIR; A59304; A59304.
DR   AlphaFoldDB; Q00407; -.
DR   SMR; Q00407; -.
DR   PRIDE; Q00407; -.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   Gene3D; 1.10.645.10; -; 1.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   Pfam; PF00374; NiFeSe_Hases; 2.
DR   SUPFAM; SSF56762; SSF56762; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Oxidoreductase.
FT   CHAIN           1..420
FT                   /note="F420-non-reducing hydrogenase vhu subunit A"
FT                   /id="PRO_0000199731"
FT   BINDING         61
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   BINDING         64
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   420 AA;  46066 MW;  6C27359F703DFC96 CRC64;
     MGKITIAPLT RLEGHGKVTI KLDDSGKPAD VKLHITALRG FEQFVIGRPA EEVPRIVPRI
     CGICQTAHHL ASVKAVDAAW GAQIPSAAEK QRELMHLGNM IHSHALHFYY LAAPDFVLGP
     DADPAIRNIV GVIDAAPEVA KKAIAMRRVG QSMVEATGGK PIHPVTGIPG GLSKSMSEEK
     RDELLAEIDT MIQYGQDGLD LMKSLNEKYL DTINSLGVID TWYLGLVKDG KHNFYGDTLR
     FVSPDGSEKM EFKPAEYLDY LGEHVVEHSY VKYPYNKKVG YPEGLYRVGP LAMINVCDSM
     STPLAEEARK EFAETFGRPA NQSIAYNQAR LIELLSACER AKELLEDPEI VSTDVKAEVE
     PKAGNGVGVV YAPRGTLFHN YETDDNGIVT KANMIVATTH NVPTMEKAIQ QAAEVLFKDN