ID   VHUU_METJA              Reviewed;          50 AA.
AC   P81335;
DT   11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 3.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=F420-non-reducing hydrogenase vhu subunit U;
DE            EC=1.12.99.-;
DE   Flags: Precursor;
GN   Name=vhuU; OrderedLocusNames=MJ1192.1;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   PROBABLE SELENOCYSTEINE AT SEC-27.
RX   PubMed=9102456; DOI=10.1006/jmbi.1996.0812;
RA   Wilting R., Schorling S., Persson B.C., Boeck A.;
RT   "Selenoprotein synthesis in archaea: identification of an mRNA element of
RT   Methanococcus jannaschii probably directing selenocysteine insertion.";
RL   J. Mol. Biol. 266:637-641(1997).
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000305};
CC   -!- SUBUNIT: The F420-non-reducing hydrogenase vhu is composed of four
CC       subunits; VhuA, VhuD, VhuG and VhuU. {ECO:0000250}.
CC   -!- MISCELLANEOUS: The large subunit of Vhu is split into VhuA and VhuU.
CC       Each contributes two ligands to the [NiFeSe] center.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB99197.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; L77117; AAB99197.1; ALT_SEQ; Genomic_DNA.
DR   STRING; 243232.MJ_1192.1; -.
DR   EnsemblBacteria; AAB99197; AAB99197; MJ_1192.1.
DR   KEGG; mja:MJ_1192.1; -.
DR   HOGENOM; CLU_3387472_0_0_2; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.645.10; -; 1.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   SUPFAM; SSF56762; SSF56762; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Oxidoreductase; Reference proteome; Selenocysteine.
FT   CHAIN           1..33
FT                   /note="F420-non-reducing hydrogenase vhu subunit U"
FT                   /id="PRO_0000013409"
FT   PROPEP          34..50
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000013410"
FT   BINDING         27
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   BINDING         30
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   NON_STD         27
FT                   /note="Selenocysteine"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   50 AA;  5726 MW;  A0BF6051D99602A0 CRC64;
     MAEKSTVKVD EVKLNLIEMV LRAYDPUYSC AAHIIVKDEK GNKIIEVIKE