ID   VHUU_METKA              Reviewed;          42 AA.
AC   P0C1V6;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=F420-non-reducing hydrogenase vhu subunit U;
DE            EC=1.12.99.-;
DE   Flags: Precursor;
GN   Name=vhuU; OrderedLocusNames=MK0177.1;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000305};
CC   -!- SUBUNIT: The F420-non-reducing hydrogenase vhu is composed of four
CC       subunits; VhuA, VhuD, VhuG and VhuU. {ECO:0000250}.
CC   -!- MISCELLANEOUS: The large subunit of Vhu is split into VhuA and VhuU.
CC       Each contributes two ligands to the [NiFeSe] center.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC       family. {ECO:0000305}.
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DR   EMBL; AE009439; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   SUPFAM; SSF56762; SSF56762; 1.
DR   PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Oxidoreductase; Reference proteome; Selenocysteine.
FT   CHAIN           1..27
FT                   /note="F420-non-reducing hydrogenase vhu subunit U"
FT                   /id="PRO_0000249594"
FT   PROPEP          28..42
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000249595"
FT   BINDING         21
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   BINDING         24
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   NON_STD         21
FT                   /note="Selenocysteine"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   42 AA;  5092 MW;  6D0614F1CD624BF1 CRC64;
     MAEKRDVLNY LEMVIRSYDI ULSCAAHVLD RVKFRIERKD ED