VIBB_VIBC3
ID VIBB_VIBC3 Reviewed; 293 AA.
AC A5F3F4; C3LYD9; O07900; Q9JQ11;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Vibriobactin-specific isochorismatase;
DE EC=3.3.2.1;
DE AltName: Full=2,3 dihydro-2,3 dihydroxybenzoate synthase;
DE AltName: Full=Isochorismate lyase-ArCP;
GN Name=vibB; OrderedLocusNames=VC0395_A0300, VC395_0788;
OS Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS O395).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=345073;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11112537; DOI=10.1021/bi001651a;
RA Keating T.A., Marshall C.G., Walsh C.T.;
RT "Vibriobactin biosynthesis in Vibrio cholerae: VibH is an amide synthase
RT homologous to nonribosomal peptide synthetase condensation domains.";
RL Biochemistry 39:15513-15521(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA Heidelberg J.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA Wang W., Wang J., Qian W., Li D., Wang L.;
RT "A recalibrated molecular clock and independent origins for the cholera
RT pandemic clones.";
RL PLoS ONE 3:E4053-E4053(2008).
CC -!- FUNCTION: Involved in the biosynthesis of the catechol siderophore
CC vibriobactin. Vibriobactin is a chelating compound involved in
CC transporting iron from the bacterial environment into the cell
CC cytoplasm (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + isochorismate = (2S,3S)-2,3-dihydroxy-2,3-
CC dihydrobenzoate + pyruvate; Xref=Rhea:RHEA:11112, ChEBI:CHEBI:15361,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:29780, ChEBI:CHEBI:58764; EC=3.3.2.1;
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000250};
CC Note=Binds 1 phosphopantetheine covalently. {ECO:0000250};
CC -!- PATHWAY: Siderophore biosynthesis; vibriobactin biosynthesis.
CC -!- SIMILARITY: Belongs to the isochorismatase family. {ECO:0000305}.
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DR EMBL; AF287252; AAG00563.1; -; Genomic_DNA.
DR EMBL; CP000627; ABQ20715.1; -; Genomic_DNA.
DR EMBL; CP001235; ACP08805.1; -; Genomic_DNA.
DR RefSeq; WP_000997093.1; NZ_JAACZH010000017.1.
DR AlphaFoldDB; A5F3F4; -.
DR SMR; A5F3F4; -.
DR STRING; 345073.VC395_0788; -.
DR EnsemblBacteria; ABQ20715; ABQ20715; VC0395_A0300.
DR GeneID; 57739481; -.
DR KEGG; vco:VC0395_A0300; -.
DR KEGG; vcr:VC395_0788; -.
DR PATRIC; fig|345073.21.peg.762; -.
DR eggNOG; COG1535; Bacteria.
DR eggNOG; COG3433; Bacteria.
DR HOGENOM; CLU_068979_2_0_6; -.
DR OMA; RDIKPFF; -.
DR UniPathway; UPA00022; -.
DR Proteomes; UP000000249; Chromosome 2.
DR GO; GO:0008908; F:isochorismatase activity; IEA:UniProtKB-EC.
DR GO; GO:0019537; P:vibriobactin biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.1200.10; -; 1.
DR Gene3D; 3.40.50.850; -; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR016291; Isochorismatase.
DR InterPro; IPR000868; Isochorismatase-like.
DR InterPro; IPR036380; Isochorismatase-like_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR Pfam; PF00857; Isochorismatase; 1.
DR Pfam; PF00550; PP-binding; 1.
DR PIRSF; PIRSF001111; Isochorismatase; 1.
DR PRINTS; PR01398; ISCHRISMTASE.
DR SUPFAM; SSF47336; SSF47336; 1.
DR SUPFAM; SSF52499; SSF52499; 1.
DR PROSITE; PS50075; CARRIER; 1.
PE 3: Inferred from homology;
KW Hydrolase; Phosphopantetheine; Phosphoprotein.
FT CHAIN 1..293
FT /note="Vibriobactin-specific isochorismatase"
FT /id="PRO_0000321856"
FT DOMAIN 211..287
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 248
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 293 AA; 32553 MW; A64283991E17A3F5 CRC64;
MAIPKIASYP LPVSLPTNKV DWRIDASRAV LLIHDMQEYF VHYFDSQAEP IPSLIKHIQQ
LKAHAKQAGI PVVYTAQPAN QDPAERALLS DFWGPGLSEE TAIIAPLAPE SGDVQLTKWR
YSAFKKSPLL DWLRETGRDQ LIITGVYAHI GILSTALDAF MFDIQPFVIG DGVADFSLSD
HEFSLRYISG RTGAVKSTQQ ACLEIAAQHS KLTGLSLRTM QHDVAAALNL SVDEVDVQEN
LLFLGLDSIR AIQLLEKWKA QGADISFAQL MEHVTLQQWW QTIQANLHQP CSA
