ID   VID27_SCHPO             Reviewed;         801 AA.
AC   Q1MTR3; O74334; Q9US95;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Vacuolar import and degradation protein 27;
GN   Name=vid27; ORFNames=SPBC1685.14c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 159-339.
RC   STRAIN=ATCC 38364 / 968;
RX   PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA   Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA   Hiraoka Y.;
RT   "Large-scale screening of intracellular protein localization in living
RT   fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL   Genes Cells 5:169-190(2000).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-405; SER-415 AND THR-478, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=18257517; DOI=10.1021/pr7006335;
RA   Wilson-Grady J.T., Villen J., Gygi S.P.;
RT   "Phosphoproteome analysis of fission yeast.";
RL   J. Proteome Res. 7:1088-1097(2008).
CC   -!- FUNCTION: Has a role in the negative regulation of gluconeogenesis.
CC       Required for vacuolar catabolite degradation of fructose-1,6-
CC       bisphosphatase (FBPase) (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC   -!- SIMILARITY: Belongs to the VID27 family. {ECO:0000305}.
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DR   EMBL; CU329671; CAA20062.2; -; Genomic_DNA.
DR   EMBL; AB027933; BAA87237.1; -; Genomic_DNA.
DR   PIR; T39530; T39530.
DR   RefSeq; NP_595218.1; NM_001021125.2.
DR   AlphaFoldDB; Q1MTR3; -.
DR   BioGRID; 276509; 6.
DR   STRING; 4896.SPBC1685.14c.1; -.
DR   iPTMnet; Q1MTR3; -.
DR   MaxQB; Q1MTR3; -.
DR   PaxDb; Q1MTR3; -.
DR   PRIDE; Q1MTR3; -.
DR   EnsemblFungi; SPBC1685.14c.1; SPBC1685.14c.1:pep; SPBC1685.14c.
DR   GeneID; 2539965; -.
DR   KEGG; spo:SPBC1685.14c; -.
DR   PomBase; SPBC1685.14c; vid27.
DR   VEuPathDB; FungiDB:SPBC1685.14c; -.
DR   eggNOG; KOG2395; Eukaryota.
DR   HOGENOM; CLU_007002_0_0_1; -.
DR   InParanoid; Q1MTR3; -.
DR   OMA; PFIITWS; -.
DR   PRO; PR:Q1MTR3; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005635; C:nuclear envelope; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR011044; Quino_amine_DH_bsu.
DR   InterPro; IPR040458; Vid27.
DR   InterPro; IPR013863; VID27_C.
DR   InterPro; IPR040979; Vid27_N.
DR   InterPro; IPR040768; Vid27_PH.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   PANTHER; PTHR31913; PTHR31913; 1.
DR   Pfam; PF08553; VID27; 1.
DR   Pfam; PF17748; VID27_N; 1.
DR   Pfam; PF17747; VID27_PH; 1.
DR   SUPFAM; SSF50969; SSF50969; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Phosphoprotein; Reference proteome.
FT   CHAIN           1..801
FT                   /note="Vacuolar import and degradation protein 27"
FT                   /id="PRO_0000339146"
FT   REGION          372..427
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        395..420
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         405
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
FT   MOD_RES         415
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
FT   MOD_RES         478
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
SQ   SEQUENCE   801 AA;  92427 MW;  90FA9BDA22DCB4ED CRC64;
     MFMLKSLGKY IWGNTNSTEI VQIPYGQLYS VHENYRECMF KDASASIRRT TAEFQYQLVI
     QRAYEEGEEE LEDDGDEAED EQSFLLDEKL HLRFDVNKDS ITIMWDNPDF QDGTLYEFTC
     ENCLQEGVAY TFEMVALQCM YERKYRQSHE KATLADLEQF SNPITRPSKE VDSLENIVTK
     LDLESEDLMR LKKQEQLDDE IAKKYLLGQQ EAEEPLVQQQ TSIVNPEKEE VTKTENIKSL
     EGELMGTISA ELHLFDAVEE VFILQDPNVE ASVFDLGDWN YWFTISTEEK TWLSQSVDAD
     MNPVFSFEHL SFIWNYFDAN SNAFSWLLRF DSQVRMEQFQ ELLMRALWES LNQQRWLKID
     DEQRDYVMET FHEDEELEDS EDEEFARQQL LSRKEEEEEE DEEASDFEDS FADFSDGEAD
     DLDESRWRKE AAKEHNSLLA VGYKNDRSYV VRNNKIGVFK HVDEKGLKFQ TALNNLSTPK
     GKSLRPSKLM LHNQDSSILF QTENAPHSLY HMDIEYGKIV DEWKVHDDVP LVTFTPDNKF
     AQMTAEQTLI GLSNNSIFRI DPRVEGNKLV AEQFKQYATK NDFSSAATTE NGYIAVASNK
     GDIRLFDRIG VNAKTALPAL GEAIIGVDVT ASGDFVLATC KTYILLIDTR IKEGRYAGRL
     GFERNFAKDK KPKPKRLQLS PQHIAMMQRE LKGGASFTPA KFNTGIDAKE TTIVSSIGPF
     LISWNLDRVK RGFTDSYKIR RYDANVQAED FRFGTDRSLI VALPDDVAMV DKSSLRRPTR
     ESICTPVKKL RSKHDIVNAP Y