ID   VIE3_MUHVS              Reviewed;         611 AA.
AC   P29832;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   02-JUN-2021, entry version 68.
DE   RecName: Full=Immediate-early protein 3;
DE            Short=IE3;
GN   Name=IE1;
OS   Murid herpesvirus 1 (strain Smith) (MuHV-1) (Mouse cytomegalovirus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Betaherpesvirinae; Muromegalovirus.
OX   NCBI_TaxID=10367;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Isolate ATCC VR-1399;
RX   PubMed=1309246; DOI=10.1128/jvi.66.1.27-36.1992;
RA   Messerle M., Buehler B., Keil G.M., Koszinowski U.H.;
RT   "Structural organization, expression, and functional characterization of
RT   the murine cytomegalovirus immediate-early gene 3.";
RL   J. Virol. 66:27-36(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8971012; DOI=10.1128/jvi.70.12.8833-8849.1996;
RA   Rawlinson W.D., Farrell H.E., Barrell B.G.;
RT   "Analysis of the complete DNA sequence of murine cytomegalovirus.";
RL   J. Virol. 70:8833-8849(1996).
CC   -!- FUNCTION: Strong transcriptional activator of the E1 promoter, shows an
CC       autoregulatory function by repression of the IE1/IE3 promoter. The IE1
CC       protein has some additive effect on the trans-activating properties of
CC       the IE3 protein.
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=IE1;
CC         IsoId=P29832-1; Sequence=Displayed;
CC       Name=IE3;
CC         IsoId=P29832-2; Sequence=Not described;
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DR   EMBL; L06816; AAA74505.1; -; Genomic_DNA.
DR   EMBL; M77846; AAA45948.1; -; Genomic_DNA.
DR   EMBL; U68299; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A40835; EDBESM.
DR   SMR; P29832; -.
DR   PRIDE; P29832; -.
DR   Proteomes; UP000008774; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039645; P:modulation by virus of host G1/S transition checkpoint; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR005028; Herpes_IE2_3.
DR   Pfam; PF03361; Herpes_IE2_3; 1.
PE   4: Predicted;
KW   Activator; Alternative splicing; Early protein;
KW   G1/S host cell cycle checkpoint dysregulation by virus; Host nucleus;
KW   Host-virus interaction; Modulation of host cell cycle by virus;
KW   Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..611
FT                   /note="Immediate-early protein 3"
FT                   /id="PRO_0000115358"
FT   REGION          119..155
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          180..383
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           141..147
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   MOTIF           279..285
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        188..202
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        209..226
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        241..260
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        261..275
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        337..377
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   611 AA;  68103 MW;  E7F10C8048536E14 CRC64;
     MEPAAPSCNM IMIADQASVN AHGRHLDENR VYPSDKVPAH VANKILESGT ETVRCDLTLE
     DMLGDYEYDD PTEEEKILMD RIADHVGNDN SDMAIKHAAG PEMNIDIVTA AASMSGITTA
     SHSALPIRRP NTPDFRRHSE KKAKKHKNKQ RQRLDDVVAK LHKPSESEAD EDFIFQQPQE
     EEYQEEQEPQ VISSSPQHFD ERSPSRSSSD HGGHSGGNSR DGQFYLSSGS EDEDDEDDER
     VDSGYRGSSR SEDSSRYRPH GGSHSSRSSI KSSGSGSSRH HHKRKAVPER HHPFTPPSAK
     RYAAAAPSSR YECPVRVDSS DSDDAPTMVQ RGTLKIKSFR PPSSGSNSNK HSSSSGGSTS
     SSHKKQQQQQ APSKKPVMSS GSDKIRDMVD RTAGGYVAPN AHKKCREDKS RKYPARALEY
     KNLPFRPQSP QYLLGKAIQF CKEETVHDKF IMLFYTRSQD VRKAVDETRA RMGMRPNLSI
     SCPFMTEHTK PINHSRETID RTSAACTAGT QAVWDMEERR GQKCVPRTSD YRSMIIQAAN
     PPDFLGAVKT CLHLSQVFPK QVCMRLCSIT GGLNPLPIYE ETVSSYVNAQ FEADDISHHE
     DESGEYESDC E