VIF_SIVGB
ID VIF_SIVGB Reviewed; 172 AA.
AC P22383;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 23-FEB-2022, entry version 84.
DE RecName: Full=Virion infectivity factor;
DE Short=Vif;
DE AltName: Full=Q protein;
DE AltName: Full=SOR protein;
GN Name=vif;
OS Simian immunodeficiency virus (isolate GB1) (SIV-mnd) (Simian
OS immunodeficiency virus mandrill).
OC Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
OC Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus.
OX NCBI_TaxID=11732;
OH NCBI_TaxID=9527; Cercopithecidae (Old World monkeys).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2797181; DOI=10.1038/341539a0;
RA Tsujimoto H., Hasegawa A., Maki N., Fukasawa M., Miura T., Speidel S.,
RA Cooper R.W., Moriyama E.N., Gojobori T., Hayami M.;
RT "Sequence of a novel simian immunodeficiency virus from a wild-caught
RT African mandrill.";
RL Nature 341:539-541(1989).
CC -!- FUNCTION: Counteracts the innate antiviral activity of APOBEC3G. Forms
CC a complex with host APOBEC3G thus preventing the entry of this lethally
CC hypermutating enzyme into progeny virions. Functions as an adapter
CC molecule, recruiting APOBEC3G to the ubiquitin-proteasome machinery.
CC Targets APOBEC3G for degradation through the assembly with elongin BC
CC complex, CUL5 and RBX1. Binds viral RNA and affects the stability of
CC viral nucleoprotein core. May play a role in viral morphology (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimer; in vitro and presumably in vivo. Interacts with
CC viral Pr55Gag precursor and host APOBEC3G. The interaction between Vif
CC and APOBEC3G is species-specific, which may play a role in restricting
CC the replication of SIV to their host. Forms an E3 ligase complex by
CC interacting with host CUL5 and elongin BC complex (ELOB and ELOC) (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}. Host cell membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Virion {ECO:0000250}. Note=Seems to colocalize with
CC intermediate filament vimentin. A fraction is associated with the
CC cytoplasmic side of cellular membranes, presumably via the interaction
CC with Pr55Gag precursor (By similarity). {ECO:0000250}.
CC -!- INDUCTION: Expressed late during infection in a Rev-dependent manner.
CC -!- DOMAIN: The BC-like-box motif mediates the interaction with elongin BC
CC complex. {ECO:0000250}.
CC -!- DOMAIN: The HCCH motif (H-x(5)-C-x(18)-C-x(5)-H) mediates the
CC interaction with CUL5. {ECO:0000250}.
CC -!- PTM: Processed in virion by the viral protease. {ECO:0000250}.
CC -!- PTM: Highly phosphorylated on serine and threonine residues.
CC {ECO:0000250}.
CC -!- PTM: Polyubiquitinated and degraded by the proteasome in the presence
CC of APOBEC3G. {ECO:0000250}.
CC -!- MISCELLANEOUS: Vif-defective viruses show catastrophic failure in
CC reverse transcription due to APOBEC-induced mutations that initiate a
CC DNA base repair pathway and compromise the structural integrity of the
CC ssDNA. In the absence of Vif, the virion is morphologically abnormal.
CC -!- MISCELLANEOUS: This is an African mandrill isolate.
CC -!- SIMILARITY: Belongs to the primate lentivirus group Vif protein family.
CC {ECO:0000305}.
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DR EMBL; M27470; AAB49570.1; -; Genomic_RNA.
DR SMR; P22383; -.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0019058; P:viral life cycle; IEA:InterPro.
DR InterPro; IPR000475; Vif.
DR Pfam; PF00559; Vif; 1.
DR PRINTS; PR00349; VIRIONINFFCT.
PE 2: Evidence at transcript level;
KW Host cell membrane; Host cytoplasm; Host membrane; Host-virus interaction;
KW Membrane; Phosphoprotein; Ubl conjugation; Ubl conjugation pathway; Virion.
FT CHAIN 1..172
FT /note="Virion infectivity factor"
FT /id="PRO_0000085330"
FT MOTIF 119..150
FT /note="HCCH motif"
FT /evidence="ECO:0000250"
FT MOTIF 157..166
FT /note="BC-box-like motif"
FT /evidence="ECO:0000250"
FT MOD_RES 107
FT /note="Phosphothreonine; by host"
FT /evidence="ECO:0000250"
FT MOD_RES 157
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000250"
SQ SEQUENCE 172 AA; 20499 MW; 205E7BDDE6265FCB CRC64;
MERVERIVRL TWKVSSQRIE KWHWLVRRQM AWATANNEEG CWWLYPHFMA YNEWYTCSKV
VIIINRDIRL IVRSYWHLQI EVGCLSTYAV SIEAVVRPPP FEKEWCTEIT PEVADHLIHL
HFYDCFMDSA VMKAIRGEEV LKVCRFPAGH KAQGVLSLQF LCLRVIYGPE ER