VIGLN_MOUSE
ID VIGLN_MOUSE Reviewed; 1268 AA.
AC Q8VDJ3;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Vigilin;
DE AltName: Full=High density lipoprotein-binding protein;
DE Short=HDL-binding protein;
GN Name=Hdlbp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=16452087; DOI=10.1074/mcp.t500041-mcp200;
RA Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.;
RT "Comprehensive identification of phosphorylation sites in postsynaptic
RT density preparations.";
RL Mol. Cell. Proteomics 5:914-922(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=18630941; DOI=10.1021/pr800223m;
RA Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.;
RT "Specific phosphopeptide enrichment with immobilized titanium ion affinity
RT chromatography adsorbent for phosphoproteome analysis.";
RL J. Proteome Res. 7:3957-3967(2008).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND SER-1252, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-991, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: Appears to play a role in cell sterol metabolism. It may
CC function to protect cells from over-accumulation of cholesterol (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
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DR EMBL; BC021765; AAH21765.1; -; mRNA.
DR EMBL; BC023806; AAH23806.1; -; mRNA.
DR EMBL; BC025648; AAH25648.1; -; mRNA.
DR EMBL; BC027779; AAH27779.1; -; mRNA.
DR EMBL; BC027788; AAH27788.1; -; mRNA.
DR EMBL; BC035301; AAH35301.1; -; mRNA.
DR CCDS; CCDS15189.1; -.
DR RefSeq; NP_001288293.1; NM_001301364.1.
DR RefSeq; NP_598569.1; NM_133808.5.
DR RefSeq; XP_006529137.1; XM_006529074.2.
DR RefSeq; XP_006529138.1; XM_006529075.2.
DR RefSeq; XP_006529139.1; XM_006529076.2.
DR RefSeq; XP_006529140.1; XM_006529077.3.
DR AlphaFoldDB; Q8VDJ3; -.
DR SMR; Q8VDJ3; -.
DR BioGRID; 225750; 22.
DR IntAct; Q8VDJ3; 4.
DR MINT; Q8VDJ3; -.
DR STRING; 10090.ENSMUSP00000127903; -.
DR iPTMnet; Q8VDJ3; -.
DR PhosphoSitePlus; Q8VDJ3; -.
DR SwissPalm; Q8VDJ3; -.
DR EPD; Q8VDJ3; -.
DR jPOST; Q8VDJ3; -.
DR MaxQB; Q8VDJ3; -.
DR PaxDb; Q8VDJ3; -.
DR PRIDE; Q8VDJ3; -.
DR ProteomicsDB; 297574; -.
DR Antibodypedia; 1371; 329 antibodies from 29 providers.
DR DNASU; 110611; -.
DR Ensembl; ENSMUST00000042498; ENSMUSP00000043047; ENSMUSG00000034088.
DR Ensembl; ENSMUST00000170883; ENSMUSP00000127903; ENSMUSG00000034088.
DR GeneID; 110611; -.
DR KEGG; mmu:110611; -.
DR UCSC; uc007cdx.2; mouse.
DR CTD; 3069; -.
DR MGI; MGI:99256; Hdlbp.
DR VEuPathDB; HostDB:ENSMUSG00000034088; -.
DR eggNOG; KOG2208; Eukaryota.
DR GeneTree; ENSGT00900000141059; -.
DR HOGENOM; CLU_008532_0_0_1; -.
DR InParanoid; Q8VDJ3; -.
DR OMA; ARSDIMN; -.
DR OrthoDB; 248989at2759; -.
DR PhylomeDB; Q8VDJ3; -.
DR TreeFam; TF323767; -.
DR Reactome; R-MMU-8964011; HDL clearance.
DR BioGRID-ORCS; 110611; 6 hits in 76 CRISPR screens.
DR ChiTaRS; Hdlbp; mouse.
DR PRO; PR:Q8VDJ3; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8VDJ3; protein.
DR Bgee; ENSMUSG00000034088; Expressed in embryonic post-anal tail and 262 other tissues.
DR ExpressionAtlas; Q8VDJ3; baseline and differential.
DR Genevisible; Q8VDJ3; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0034364; C:high-density lipoprotein particle; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005844; C:polysome; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1370.10; -; 13.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR004088; KH_dom_type_1.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR Pfam; PF00013; KH_1; 14.
DR SMART; SM00322; KH; 14.
DR SUPFAM; SSF54791; SSF54791; 14.
DR PROSITE; PS50084; KH_TYPE_1; 14.
PE 1: Evidence at protein level;
KW Acetylation; Cholesterol metabolism; Cytoplasm; HDL; Lipid metabolism;
KW Lipid transport; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW RNA-binding; Steroid metabolism; Sterol metabolism; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q00341"
FT CHAIN 2..1268
FT /note="Vigilin"
FT /id="PRO_0000050132"
FT DOMAIN 150..212
FT /note="KH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 222..284
FT /note="KH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 295..357
FT /note="KH 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 364..424
FT /note="KH 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 435..497
FT /note="KH 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 507..570
FT /note="KH 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 581..643
FT /note="KH 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 653..716
FT /note="KH 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 727..790
FT /note="KH 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 800..863
FT /note="KH 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 873..967
FT /note="KH 11"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 972..1034
FT /note="KH 12"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 1052..1117
FT /note="KH 13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT DOMAIN 1127..1190
FT /note="KH 14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00117"
FT REGION 910..947
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1213..1268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 928..947
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1230..1252
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:Q00341"
FT MOD_RES 8
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q00341"
FT MOD_RES 11
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q00341"
FT MOD_RES 31
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:18630941, ECO:0007744|PubMed:21183079"
FT MOD_RES 295
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 296
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 317
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q00341"
FT MOD_RES 437
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q00341"
FT MOD_RES 645
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Z1A6"
FT MOD_RES 991
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 1247
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q00341"
FT MOD_RES 1252
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 1268 AA; 141743 MW; 2502F3D4E9EE230C CRC64;
MSSVAVLTQE SFAEHRSGLV PQQIKVATLN SEEENDPPTY KDAFPPLPEK AACLESAQEP
AGAWSNKIRP IKASVITQVF HVPLEERKYK DMNQFGEGEQ AKICLEIMQR TGAHLELSLA
KDQGLSIMVS GKLDAVMKAR KDIVARLQTQ ASATVPIPKE HHRFVIGKNG EKLQDLELKT
ATKIQIPRPD DPSNQIKITG TKEGIEKARH EVLLISAEQD KRAVERLEVE KAFHPFIAGP
YNRLVGEIMQ ETGTRINIPP PSVNRTEIVF TGEKEQLAQA VARIKKIYEE KKKKTTTIAV
EVKKSQHKYV IGPKGNSLQE ILERTGVSVE IPPSDSISET VILRGEPEKL GQALTEVYAK
ANSFTVSSVS APSWLHRFII GKKGQNLAKI TQQMPKVHIE FTEGEDKITL EGPTEDVNVA
QEQIEGMVKD LINRMDYVEI NIDHKFHRHL IGKSGANINR IKDQYKVSVR IPPDSEKSNL
IRIEGDPQGV QQAKRELLEL ASRMENERTK DLIIEQRFHR TIIGQKGERI REIRDKFPEV
IINFPDPAQK SDIVQLRGPK NEVEKCTKYM QKMVADLVEN SYSISVPIFK QFHKNIIGKG
GANIKKIREE SNTKIDLPAE NSNSETIIIT GKRANCEAAR SRILSIQKDL ANIAEVEVSI
PAKLHNSLIG TKGRLIRSIM EECGGVHIHF PVEGSGSDTV VIRGPSSDVE KAKKQLLHLA
EEKQTKSFTV DIRAKPEYHK FLIGKGGGKI RKVRDSTGAR IIFPAAEDKD QDLITIIGKE
DAVREAQKEL EALIQNLENV VEDYMLVDPK HHRHFVIRRG QVLREIAEEY GGVMVSFPRS
GTQSDKVTLK GAKDCVEAAK KRIQEIIEDL EAQVTVECAI PQKFHRSVMG PKGSRIQQIT
RDYNVQIKFP DREENPVHSV EPSIQENGDE AGEGREAKET DPGSPRRCDI IIISGRKEKC
EAAKEALEAL VPVTIEVEVP FDLHRYIIGQ KGSGIRKMMD EFEVNIHVPA PELQSDTIAI
TGLAANLDRA KAGLLDRVKE LQAEQEDRAL RSFKLSVTVD PKYHPKIIGR KGAVITQIRL
EHEVNIQFPD KDDGNQPQDQ ITITGYEKNT EAARDAILKI VGELEQMVSE DVPLDHRVHA
RIIGARGKAI RKIMDEFKVD IRFPQSGAPD PNCVTVTGLP ENVEEAIDHI LNLEEEYLAD
VVDSEALQVY MKPPAHEESR APSKGFVVRD APWTSNSSEK APDMSSSEEF PSFGAQVAPK
TLPWGPKR
