ID   VILB_DICDI              Reviewed;         959 AA.
AC   P36418; Q551N4;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 125.
DE   RecName: Full=Protovillin;
DE   AltName: Full=100 kDa actin-binding protein;
GN   Name=vilB; ORFNames=DDB_G0276453;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 569-586 AND
RP   843-853.
RC   STRAIN=AX2, and AX3;
RX   PubMed=8344435; DOI=10.1016/0014-5793(93)80968-z;
RA   Hofmann A., Noegel A.A., Bomblies L., Lottspeich F., Schleicher M.;
RT   "The 100 kDa F-actin capping protein of Dictyostelium amoebae is a villin
RT   prototype ('protovillin').";
RL   FEBS Lett. 328:71-76(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Caps actin filaments but displays neither severing nor cross-
CC       linking nor nucleating activities. Protovillin seems to be a villin
CC       precursor with only archaic capping activity. It lacks essential
CC       changes in the sequence to allow bundling of actin filaments and
CC       consequently the appearance of microvilli.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC   -!- DEVELOPMENTAL STAGE: Present in vegetative and developing cells.
CC   -!- SIMILARITY: Belongs to the villin/gelsolin family. {ECO:0000305}.
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DR   EMBL; X74387; CAA52410.1; -; Genomic_DNA.
DR   EMBL; AAFI02000015; EAL69180.1; -; Genomic_DNA.
DR   PIR; S35061; S35061.
DR   RefSeq; XP_643085.1; XM_637993.1.
DR   AlphaFoldDB; P36418; -.
DR   SMR; P36418; -.
DR   STRING; 44689.DDB0185072; -.
DR   PaxDb; P36418; -.
DR   EnsemblProtists; EAL69180; EAL69180; DDB_G0276453.
DR   GeneID; 8620489; -.
DR   KEGG; ddi:DDB_G0276453; -.
DR   dictyBase; DDB_G0276453; vilB.
DR   eggNOG; KOG0443; Eukaryota.
DR   HOGENOM; CLU_002568_3_1_1; -.
DR   InParanoid; P36418; -.
DR   OMA; DPNIWSA; -.
DR   PhylomeDB; P36418; -.
DR   Reactome; R-DDI-6798695; Neutrophil degranulation.
DR   PRO; PR:P36418; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR   GO; GO:0003785; F:actin monomer binding; IDA:dictyBase.
DR   GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IBA:GO_Central.
DR   GO; GO:0051014; P:actin filament severing; IBA:GO_Central.
DR   GO; GO:0008154; P:actin polymerization or depolymerization; IBA:GO_Central.
DR   GO; GO:0051016; P:barbed-end actin filament capping; IDA:dictyBase.
DR   Gene3D; 1.10.950.10; -; 1.
DR   Gene3D; 3.40.20.10; -; 6.
DR   InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR   InterPro; IPR007123; Gelsolin-like_dom.
DR   InterPro; IPR007122; Villin/Gelsolin.
DR   InterPro; IPR003128; Villin_headpiece.
DR   InterPro; IPR036886; Villin_headpiece_dom_sf.
DR   PANTHER; PTHR11977; PTHR11977; 1.
DR   Pfam; PF00626; Gelsolin; 5.
DR   Pfam; PF02209; VHP; 1.
DR   PRINTS; PR00597; GELSOLIN.
DR   SMART; SM00262; GEL; 6.
DR   SMART; SM00153; VHP; 1.
DR   SUPFAM; SSF47050; SSF47050; 1.
DR   PROSITE; PS51089; HP; 1.
PE   1: Evidence at protein level;
KW   Actin capping; Actin-binding; Cytoplasm; Cytoskeleton;
KW   Direct protein sequencing; Reference proteome; Repeat.
FT   CHAIN           1..959
FT                   /note="Protovillin"
FT                   /id="PRO_0000218731"
FT   REPEAT          64..116
FT                   /note="Gelsolin-like 1"
FT   REPEAT          204..244
FT                   /note="Gelsolin-like 2"
FT   REPEAT          309..366
FT                   /note="Gelsolin-like 3"
FT   REPEAT          479..529
FT                   /note="Gelsolin-like 4"
FT   REPEAT          603..647
FT                   /note="Gelsolin-like 5"
FT   REPEAT          713..754
FT                   /note="Gelsolin-like 6"
FT   REPEAT          840..849
FT                   /note="1"
FT   REPEAT          851..860
FT                   /note="2"
FT   DOMAIN          895..959
FT                   /note="HP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00595"
FT   REGION          1..53
FT                   /note="Tail"
FT   REGION          54..832
FT                   /note="Core"
FT   REGION          840..860
FT                   /note="2 X 10 AA repeats of T-P-K-P-I-T-T-P-T-V"
SQ   SEQUENCE   959 AA;  109462 MW;  0A4716EE16E52801 CRC64;
     MEPPLELPTQ RKRVIPSKFG ILKRNAEIEA EKNRENLQQS SCFSHINEIG KEIGLEIWKI
     IDDSTIQKVP KVNHSTFETN KSYLLLMGQF YDGNMNIKTY NIHFWIGELL INSQETINFC
     NDRIEELERI IKYNQKQFDS EQFYPEPILY REFQGKEGDI FMSYFKSYGG PRYVAPLKLT
     SASAAIATAA KQYKLFHLKG RRNIRVKQVD ISSKSLNSGD VFVLDCEDFI YQWNGSESSR
     LEKGKGLDLT IRLRDEKSAK AKIIVMDEND TDKDHPEFWK RLGGCKDDVQ KAEQGGDDFA
     YEKKSVEQIK LYQVENLNYE VHLHLIDPIG DVYSTTQLNA EFCYILDCET ELYVWLGKAS
     ANDQRTVAMA NAMDLLHEDN RPSWTPIIKM TQGSENTLFK DKFKKGSWGE YVNDNFEKKP
     ITGKGVAAKA VQEKINVDAL HNPEKYQLSK EERKSTIPTL HHVDDKHRGE LKIWHVRNRN
     KFEISQSEFG LFYNQSCYLV LFTLFAADGS NNSILYYWQG RFSSSEDKGA AALLAKDVGK
     ELHRSCIHVR TVQNKEPNHF LEHFQGRMVV FKGSRPNATT EVSLENLSSS LQGLYHVRGT
     EPINIHSIQV EKAISSLDSN DSFILVNFKN TISYIWVGKY SDEKEAALQI SSNVFTGYNF
     QLIDEGDETS EFWESLETNS SLSLLKDYYT QLRTVEQEKK TRLFQCSNNS GVFKVFEIHD
     FSQDDLDSDD VMILDNQKQI FVWVGKESSD TEKLMANETA LEYIMNAPTH RRDDPIFTIQ
     DGFEPHEFTF NFHAWQVNKT QQDSYKSKLS AILGSNNSGP ASPIMLPTSG VTLKPTTAAT
     PKPITTPTVT TPKPITTPTV ATLKTVTPAV TLKPTTVTTP SKVATTTNTS TPSPTTITTF
     YPLSVLKQKT NLPNDIDKSC LHLYLSDEEF LSTFKMTKEI FQKTPAWKTK QLRVDNGLF