VILD_DICDI
ID VILD_DICDI Reviewed; 1704 AA.
AC Q8WQ85; P90524; Q54IR2;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Villidin;
GN Name=vilA; ORFNames=DDB_G0288557;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=AX2;
RX PubMed=12857859; DOI=10.1091/mbc.e02-12-0827;
RA Gloss A., Rivero F., Khaire N., Mueller R., Loomis W.F., Schleicher M.,
RA Noegel A.A.;
RT "Villidin, a novel WD-repeat and villin-related protein from Dictyostelium,
RT is associated with membranes and the cytoskeleton.";
RL Mol. Biol. Cell 14:2716-2727(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1444-1704.
RC STRAIN=AX4;
RA Loomis W.F., Iranfar N.;
RL Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May function as a linker between membranes and the actin
CC cytoskeleton. {ECO:0000269|PubMed:12857859}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:12857859};
CC Peripheral membrane protein {ECO:0000269|PubMed:12857859}. Cytoplasm,
CC cytoskeleton {ECO:0000269|PubMed:12857859}. Note=Associated with
CC membranes and the cytoskeleton.
CC -!- DEVELOPMENTAL STAGE: Present in low amounts during growth and early
CC aggregation, but increase during development and reach its highest
CC level at the tipped mound stage (at protein level).
CC {ECO:0000269|PubMed:12857859}.
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DR EMBL; AJ427856; CAD20809.1; -; Genomic_DNA.
DR EMBL; AAFI02000117; EAL63138.1; -; Genomic_DNA.
DR EMBL; U78754; AAB36957.1; -; mRNA.
DR RefSeq; XP_636652.1; XM_631560.1.
DR AlphaFoldDB; Q8WQ85; -.
DR SMR; Q8WQ85; -.
DR STRING; 44689.DDB0191538; -.
DR PaxDb; Q8WQ85; -.
DR PRIDE; Q8WQ85; -.
DR EnsemblProtists; EAL63138; EAL63138; DDB_G0288557.
DR GeneID; 8626698; -.
DR KEGG; ddi:DDB_G0288557; -.
DR dictyBase; DDB_G0288557; vilA.
DR eggNOG; KOG0303; Eukaryota.
DR eggNOG; KOG0443; Eukaryota.
DR HOGENOM; CLU_240788_0_0_1; -.
DR InParanoid; Q8WQ85; -.
DR OMA; RIFVWAG; -.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:Q8WQ85; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:dictyBase.
DR GO; GO:0005794; C:Golgi apparatus; IDA:dictyBase.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0051014; P:actin filament severing; IBA:GO_Central.
DR GO; GO:0008154; P:actin polymerization or depolymerization; IBA:GO_Central.
DR GO; GO:0051016; P:barbed-end actin filament capping; IBA:GO_Central.
DR GO; GO:0046956; P:positive phototaxis; IMP:dictyBase.
DR GO; GO:0051707; P:response to other organism; TAS:dictyBase.
DR Gene3D; 1.10.950.10; -; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR Gene3D; 3.40.20.10; -; 5.
DR InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR InterPro; IPR015048; DUF1899.
DR InterPro; IPR007123; Gelsolin-like_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR007122; Villin/Gelsolin.
DR InterPro; IPR003128; Villin_headpiece.
DR InterPro; IPR036886; Villin_headpiece_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR11977; PTHR11977; 1.
DR Pfam; PF08953; DUF1899; 1.
DR Pfam; PF00626; Gelsolin; 4.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF02209; VHP; 1.
DR Pfam; PF00400; WD40; 1.
DR PRINTS; PR00597; GELSOLIN.
DR SMART; SM01166; DUF1899; 1.
DR SMART; SM00262; GEL; 5.
DR SMART; SM00233; PH; 3.
DR SMART; SM00153; VHP; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF47050; SSF47050; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS51089; HP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 2.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Membrane; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..1704
FT /note="Villidin"
FT /id="PRO_0000051331"
FT REPEAT 82..122
FT /note="WD 1"
FT REPEAT 133..173
FT /note="WD 2"
FT REPEAT 180..221
FT /note="WD 3"
FT REPEAT 225..271
FT /note="WD 4"
FT DOMAIN 459..563
FT /note="PH 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 727..828
FT /note="PH 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 871..969
FT /note="PH 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REPEAT 1025..1119
FT /note="Gelsolin-like 1"
FT REPEAT 1138..1241
FT /note="Gelsolin-like 2"
FT REPEAT 1293..1390
FT /note="Gelsolin-like 3"
FT REPEAT 1404..1494
FT /note="Gelsolin-like 4"
FT REPEAT 1520..1615
FT /note="Gelsolin-like 5"
FT DOMAIN 1641..1704
FT /note="HP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00595"
FT REGION 439..460
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 606..721
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 848..877
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 6
FT /note="I -> F (in Ref. 1; CAD20809)"
FT /evidence="ECO:0000305"
FT CONFLICT 389
FT /note="S -> Y (in Ref. 1; CAD20809)"
FT /evidence="ECO:0000305"
FT CONFLICT 500
FT /note="Q -> P (in Ref. 1; CAD20809)"
FT /evidence="ECO:0000305"
FT CONFLICT 538
FT /note="G -> R (in Ref. 1; CAD20809)"
FT /evidence="ECO:0000305"
FT CONFLICT 541
FT /note="H -> L (in Ref. 1; CAD20809)"
FT /evidence="ECO:0000305"
FT CONFLICT 557..559
FT /note="SIN -> AIK (in Ref. 1; CAD20809)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1704 AA; 191191 MW; 1B154563964E5234 CRC64;
MEYKSIEKSI VRESKVRNVF SKVCKKELFY TNLKVNSASE ELLKSSKSLF AYPSSIGGGS
CLSITKLSNH GKVPDTPFCI KGHTDPISCF EFSNFNDYVI ATGSRDCTIK IWEVPEEGLI
KDNLVTPLIT LPKQQKRVTG VYFHPSVDSL FMSSTLDYTL DIWDLSSTGE QSSVIQKLTG
HEDLIMSVGW NTWSGGDKLA TSSRDKKMRL YDPRSQSTPI QTISTHEGAQ GFKLCWADSN
GLELICTVGA NKSAQRQLYL WDPRQLQSSQ PLVSKDVTTD SSILSPYYDF GTNMVYLGGK
GDGIYYYEIE KNNAHHIGKA TFGNVTQSAI TLLPKSVVDV NICEIDRFLR LTAANSVEMV
SFMCPRKSQL FQEDIFPPCP SGDPTTDCSL WFADKQLKVI PHTISMKPDG VQSIYEVSEE
EGGKSKEKDK LEQLSKLSIG NSGSGGGGGD GDGNGGDSPF ITEGIVKQEI EGWLFNSYEN
RYLKIVKDKI YCFLNEDSAQ AIWDSPVLNI KYVDIYDEDE NTSGEEWSLR FNIILLNGKE
HRMECSTVKQ RDAWCQSINA YREMKQQVDQ SVVSPTMGEF HLVNTTPVAP SPMVISENKN
ALQKFSVPSP KEQQQQQQQG SESPNSTTPK SSTPTQTRHH SVLSRNPSYT SLKSSGGFSK
PSPSSSTSTP PSTFASPLSI AQTSSTTTTT TTTTTTTSSS STPPPLRANN STSSTPLNSS
TGIKQSDIVI EGNLTELIPG LLWNSNTEKW YVVSEGMLYS YKNKSLKQLD PLETIHLEKA
ISAHKTKEIF TIQGFSFQLS TPNRIIHLLA KTKEERGSWL SVLRQNLKSS GETKPKSVLT
RASTMDELIS SPMSDDESNT NEGGVEEEEE EQPLEGQLSR KLPGIFSMWG QCFVALLAED
LFVSRNKLST TPELRIQLSS ISLIKKISPN EFTLYDSMNQ VVCNFRTIAP TAEFDDCTRW
IDGLEAARKR SIDIIKMFGI NEKEVLSPLP SSSSIPSSGS NGNLLDPSAD DLTRYLDLTA
IKNGKQKILI QVKGKRKIRV RMVKLSTSSL NCHNSFILDA GPRIFVWAGS KTSRVNKAKA
LDFANRIRTK ERGGKSTLIQ LDQGRDEQSF DFWEILGGNS SDPIATTPTP EEQDTESIKT
TIYRVGLDVK KNSLRARLAW EGTDWKLPNK EILNTKFVYV VDCVTEIFVW VGKESSSIQR
KMATKVALVL QAQKDRVDWT KITRLTEFGE NNLFKEKFAN YPGMLPISTT RQEIKSHVAT
SKVEHKIETL ASRMTTPFID NEALFTIPID EGGRVKVWKI EDYEKIDHPQ HLYSQFFSSD
SYIVLYTYMQ NNKEAHVIYY YLGRDSSINE KGTSAYLTVD LNESLVGACV QTRVVANKEC
KNFLNLFKTK MVIHKGKYNN YDPKKPSLYQ VKGLDKIDIR AVQVEFSSSM LNTLHVSILR
TPEKIYIWHG KFSLDAEQNS ALSIAENFNS TSAPIEILKE GSESNEFWSA FESTGGRQKY
FNDIMIQSSS IPTSFTYKPR FFVCSNASGI VEVTEESPFS QDDLDIGSVC ILDVQSHIYL
WIGSRATHRT KRASMEVVLN FIETSKLGHS KEHTKVLIAT PFEEPIGFKS YFRAWCTSKY
PKNKLPLVEK DGIPVEQVLK DYLKEIYTYE ELLADPLPAG VDSTKLDTYL NDEDFEKVFK
MTRTEWLKIP AWKREGIKKE LFLF