VILL_MOUSE
ID VILL_MOUSE Reviewed; 859 AA.
AC Q91YD6; P97809; Q8BIS5; Q8R211; Q8VDI8;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Villin-like protein;
DE AltName: Full=EF-6;
GN Name=Vill {ECO:0000312|MGI:MGI:1201781};
GN Synonyms=Villp {ECO:0000312|MGI:MGI:1201781};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAC69079.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=BALB/cJ {ECO:0000312|EMBL:CAC69079.1};
RA Protopopov A., Kashuba V., Zabarovsky E.;
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAH21808.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=Czech II {ECO:0000312|EMBL:AAH21808.1}, and
RC FVB/N {ECO:0000312|EMBL:AAH22664.1};
RC TISSUE=Kidney {ECO:0000312|EMBL:AAH22664.1}, and
RC Mammary tumor {ECO:0000312|EMBL:AAH21808.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 652-727 (ISOFORMS 1/3), AND DEVELOPMENTAL
RP STAGE.
RX PubMed=9032278; DOI=10.1128/mcb.17.3.1503;
RA Fu X., Kamps M.P.;
RT "E2a-Pbx1 induces aberrant expression of tissue-specific and
RT developmentally regulated genes when expressed in NIH 3T3 fibroblasts.";
RL Mol. Cell. Biol. 17:1503-1512(1997).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Possible tumor suppressor. {ECO:0000250|UniProtKB:O15195}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q91YD6-1; Sequence=Displayed;
CC Name=2 {ECO:0000305};
CC IsoId=Q91YD6-2; Sequence=VSP_051691, VSP_051692;
CC Name=3 {ECO:0000305};
CC IsoId=Q91YD6-3; Sequence=VSP_051690;
CC -!- DEVELOPMENTAL STAGE: Not detected in adult tissues. Expressed at low
CC levels in early embryogenesis. {ECO:0000269|PubMed:9032278}.
CC -!- SIMILARITY: Belongs to the villin/gelsolin family. {ECO:0000255}.
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DR EMBL; AJ344341; CAC69079.1; -; mRNA.
DR EMBL; AK028229; BAC25828.1; -; mRNA.
DR EMBL; BC021808; AAH21808.1; -; mRNA.
DR EMBL; BC022664; AAH22664.1; -; mRNA.
DR EMBL; U72681; AAB51041.1; -; mRNA.
DR CCDS; CCDS23606.1; -. [Q91YD6-2]
DR CCDS; CCDS52962.1; -. [Q91YD6-1]
DR RefSeq; NP_001158039.1; NM_001164567.1.
DR RefSeq; NP_035830.2; NM_011700.2.
DR AlphaFoldDB; Q91YD6; -.
DR SMR; Q91YD6; -.
DR BioGRID; 204523; 5.
DR STRING; 10090.ENSMUSP00000061731; -.
DR iPTMnet; Q91YD6; -.
DR PhosphoSitePlus; Q91YD6; -.
DR jPOST; Q91YD6; -.
DR MaxQB; Q91YD6; -.
DR PaxDb; Q91YD6; -.
DR PRIDE; Q91YD6; -.
DR ProteomicsDB; 297597; -. [Q91YD6-1]
DR ProteomicsDB; 297598; -. [Q91YD6-2]
DR ProteomicsDB; 297599; -. [Q91YD6-3]
DR Antibodypedia; 48930; 98 antibodies from 21 providers.
DR DNASU; 22351; -.
DR Ensembl; ENSMUST00000141185; ENSMUSP00000116546; ENSMUSG00000038775. [Q91YD6-3]
DR GeneID; 22351; -.
DR KEGG; mmu:22351; -.
DR CTD; 50853; -.
DR MGI; MGI:1201781; Vill.
DR VEuPathDB; HostDB:ENSMUSG00000038775; -.
DR eggNOG; KOG0443; Eukaryota.
DR GeneTree; ENSGT00940000160253; -.
DR InParanoid; Q91YD6; -.
DR OrthoDB; 1376537at2759; -.
DR PhylomeDB; Q91YD6; -.
DR BioGRID-ORCS; 22351; 4 hits in 72 CRISPR screens.
DR PRO; PR:Q91YD6; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q91YD6; protein.
DR Bgee; ENSMUSG00000038775; Expressed in granulocyte and 163 other tissues.
DR ExpressionAtlas; Q91YD6; baseline and differential.
DR Genevisible; Q91YD6; MM.
DR GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0051014; P:actin filament severing; IBA:GO_Central.
DR GO; GO:0008154; P:actin polymerization or depolymerization; IBA:GO_Central.
DR GO; GO:0051016; P:barbed-end actin filament capping; IBA:GO_Central.
DR Gene3D; 1.10.950.10; -; 1.
DR Gene3D; 3.40.20.10; -; 6.
DR InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR InterPro; IPR007123; Gelsolin-like_dom.
DR InterPro; IPR036180; Gelsolin-like_dom_sf.
DR InterPro; IPR015626; Villin-like.
DR InterPro; IPR007122; Villin/Gelsolin.
DR InterPro; IPR003128; Villin_headpiece.
DR InterPro; IPR036886; Villin_headpiece_dom_sf.
DR PANTHER; PTHR11977; PTHR11977; 1.
DR PANTHER; PTHR11977:SF30; PTHR11977:SF30; 1.
DR Pfam; PF00626; Gelsolin; 5.
DR Pfam; PF02209; VHP; 1.
DR PRINTS; PR00597; GELSOLIN.
DR SMART; SM00262; GEL; 6.
DR SMART; SM00153; VHP; 1.
DR SUPFAM; SSF47050; SSF47050; 1.
DR SUPFAM; SSF82754; SSF82754; 2.
DR PROSITE; PS51089; HP; 1.
PE 1: Evidence at protein level;
KW Actin capping; Actin-binding; Alternative splicing; Calcium;
KW Reference proteome; Repeat.
FT CHAIN 1..859
FT /note="Villin-like protein"
FT /id="PRO_0000218739"
FT REPEAT 24..76
FT /note="Gelsolin-like 1"
FT /evidence="ECO:0000255"
FT REPEAT 148..188
FT /note="Gelsolin-like 2"
FT /evidence="ECO:0000255"
FT REPEAT 264..308
FT /note="Gelsolin-like 3"
FT /evidence="ECO:0000255"
FT REPEAT 401..450
FT /note="Gelsolin-like 4"
FT /evidence="ECO:0000255"
FT REPEAT 521..561
FT /note="Gelsolin-like 5"
FT /evidence="ECO:0000255"
FT REPEAT 624..665
FT /note="Gelsolin-like 6"
FT /evidence="ECO:0000255"
FT DOMAIN 793..859
FT /note="HP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00595"
FT VAR_SEQ 1..384
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_051690"
FT VAR_SEQ 651..712
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_051691"
FT VAR_SEQ 737..758
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_051692"
FT CONFLICT 59
FT /note="S -> F (in Ref. 2; BAC25828)"
FT /evidence="ECO:0000305"
FT CONFLICT 73
FT /note="E -> K (in Ref. 2; BAC25828)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="C -> S (in Ref. 3; AAH21808)"
FT /evidence="ECO:0000305"
FT CONFLICT 128
FT /note="F -> L (in Ref. 3; AAH21808)"
FT /evidence="ECO:0000305"
FT CONFLICT 143
FT /note="K -> R (in Ref. 2; BAC25828)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="L -> V (in Ref. 3; AAH21808)"
FT /evidence="ECO:0000305"
FT CONFLICT 304
FT /note="P -> S (in Ref. 3; AAH21808)"
FT /evidence="ECO:0000305"
FT CONFLICT 375
FT /note="T -> N (in Ref. 2; BAC25828)"
FT /evidence="ECO:0000305"
FT CONFLICT 407
FT /note="H -> D (in Ref. 3; AAH21808)"
FT /evidence="ECO:0000305"
FT CONFLICT 410
FT /note="Y -> H (in Ref. 3; AAH21808)"
FT /evidence="ECO:0000305"
FT CONFLICT 568
FT /note="S -> T (in Ref. 3; AAH21808)"
FT /evidence="ECO:0000305"
FT CONFLICT 621
FT /note="A -> T (in Ref. 3; AAH21808)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 859 AA; 96509 MW; DA126D0FD75FCA75 CRC64;
MDINQDLPAI DSHRALQIWI TENLKMLPLP ERAHGNFFEE CCYVVLHVPQ SPKATQGGSS
DLHYWIGKDA SAEAREAAVS FVQCLQEDLG DQTVLHRESQ GHESDCFHSY FHPGVIYRKG
GRDSALKFAE TNMYNVQRLL HIKGRKHVSA TEVALSWNSF NKGDIFLLDL GKVMIQWNGP
KASISEKARA LTLTCNLRDR ERGGRAQIAV VDAENEATNL LRIMEAVLGC RSGSLCPSVP
SNSVSQLQKA NVRLYHVCEK GTDLVVQELA TRPLTQDLLQ EDGCYLLDQG GFKIYMWQGR
KSSPQEKKAA LSRAVGFIQA KGYPNYTNVE VVNDGAESTA FQQLFWSWSK ELDRKKHPEK
SKLVQGNLEV GKLHTQPELA AQLRMVDDGS GKVEVWYIQD LQRQPVHPKY YGQLCSGNCY
LVLYTYQKLG CVQYLLYLWQ GHQSTVEDTK ALNCSAEELD LMHQGALAQG HVTMGSEPPH
FLAIFQGRLV VFQGNAGNKG ERPPVSDTRL FHVQGTESHN TRTMEVPARA SSLTSGDVFF
LITSHVCYLW FGKGCHGDQR EMARTVVSVF PGNNKETVLE GQEPLYFWEA LGGRAPYPSN
KRLPEEVWSI QPRLFECSSH AGCLVLTEVL FFGQEDLDKY DIMLLDTCQE IFLWLGEAAG
EWKKEAVAWG LEYLRTHPAE RSLATPIFVV KQGHEPATFT GWFVTWDPYK WMNSQSYEEM
VGNSLGPGSA ISEMTAEVHN FQLTPRLSDN KAGHPALQAF KGSQDSPENE LGLDLRVDGA
NPSMNHTSSC SDSMVNGSLP RERLMHQALE DLPPGVDPAR KEFYLSDSDF QDIFGKSKEE
FYSMAKWKQQ QAKKKLGFF
