ID   VINT_BP186              Reviewed;         336 AA.
AC   P06723;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   02-DEC-2020, entry version 101.
DE   RecName: Full=Integrase;
DE            EC=2.7.7.- {ECO:0000250|UniProtKB:P03700};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:P03700};
GN   Name=int;
OS   Escherichia phage 186 (Bacteriophage 186).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Peduovirinae; Eganvirus.
OX   NCBI_TaxID=29252;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=186CITSP;
RX   PubMed=3806670; DOI=10.1016/0022-2836(86)90257-3;
RA   Kalionis B., Dodd I.B., Egan J.B.;
RT   "Control of gene expression in the P2-related template coliphages. III. DNA
RT   sequence of the major control region of phage 186.";
RL   J. Mol. Biol. 191:199-209(1986).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination. In conjunction with
CC       excisionase, integrase is also necessary for excision of the prophage
CC       from the host genome.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; U32222; AAC34175.1; -; Genomic_DNA.
DR   PIR; S09532; S09532.
DR   RefSeq; NP_052278.1; NC_001317.1.
DR   SMR; P06723; -.
DR   GeneID; 1262438; -.
DR   KEGG; vg:1262438; -.
DR   Proteomes; UP000000369; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   Pfam; PF00589; Phage_integrase; 2.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Hydrolase;
KW   Reference proteome; Transferase; Viral genome integration;
KW   Virus entry into host cell.
FT   CHAIN           1..336
FT                   /note="Integrase"
FT                   /id="PRO_0000197522"
FT   DOMAIN          55..143
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          165..325
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        203
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        226
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        277
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        280
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        303
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        312
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   336 AA;  38970 MW;  D7FB3FC22978CC55 CRC64;
     MTVRKNPAGG WICELYPNGA KGKRIRKKFA TKGEALAFEQ YTVQNPWQEE KEDRRTLKEL
     VDSWYSAHGI TLKDGLKRQL AMHHAFECMG EPLARDFDAQ MFSRYREKRL KGEYARSNRV
     KEVSPRTLNL ELAYFRAVFN ELNRLGEWKG ENPLKNMRPF RTEEMEMTWL THDQISQLLG
     ECNRHDHPDL ETVVRICLAT GARWSEAESL RKSQLAKYKI TYTNTKGRKN RTVPISKELY
     ESLPDDKKGR LFSDCYGAFR SALERTGIEL PAGQLTHVLR HTFASHFMMN GGNILVLQRV
     LGHTDIKMTM RYAHFAPDHL EDAVKLNPLV HITNSK