ID   VINT_BPHK0              Reviewed;         357 AA.
AC   P16407;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   29-SEP-2021, entry version 103.
DE   RecName: Full=Integrase;
DE            EC=2.7.7.- {ECO:0000250|UniProtKB:P03700};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:P03700};
GN   Name=int;
OS   Escherichia phage HK022 (Bacteriophage HK022).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Hendrixvirinae; Shamshuipovirus.
OX   NCBI_TaxID=10742;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2547971; DOI=10.1016/0022-2836(89)90238-6;
RA   Yagil E., Dolev S., Oberto J., Kislev N., Ramaiah N., Weisberg R.A.;
RT   "Determinants of site-specific recombination in the lambdoid coliphage
RT   HK022. An evolutionary change in specificity.";
RL   J. Mol. Biol. 207:695-717(1989).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination. In conjunction with
CC       excisionase, integrase is also necessary for excision of the prophage
CC       from the host genome.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X51962; CAA36221.1; -; Genomic_DNA.
DR   PIR; S04990; S04990.
DR   RefSeq; NP_037686.1; NC_002166.1.
DR   SMR; P16407; -.
DR   GeneID; 1262484; -.
DR   KEGG; vg:1262484; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008907; F:integrase activity; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR016177; DNA-bd_dom_sf.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR015094; Integrase_lambda-typ_DNA-bd_N.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   Pfam; PF09003; Arm-DNA-bind_1; 1.
DR   Pfam; PF02899; Phage_int_SAM_1; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF54171; SSF54171; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Hydrolase; Transferase;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..357
FT                   /note="Integrase"
FT                   /id="PRO_0000197523"
FT   DOMAIN          74..155
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          175..355
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        212
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        235
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        308
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        311
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        333
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        342
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   357 AA;  40358 MW;  7A315A7FA8F23243 CRC64;
     MGRRRSHERR DLPPNLYIRN NGYYCYRDPR TGKEFGLGRD RRIAITEAIQ ANIELLSGNR
     RESLIDRIKG ADAITLHAWL DRYETILSER GIRPKTLLDY ASKIRAIRRK LPDKPLADIS
     TKEVAAMLNT YVAEGKSASA KLIRSTLVDV FREAIAEGHV ATNPVTATRT AKSEVRRSRL
     TANEYVAIYH AAEPLPIWLR LAMDLAVVTG QRVGDLCRMK WSDINDNHLH IEQSKTGAKL
     AIPLTLTIDA LNISLADTLQ QCREASSSET IIASKHHDPL SPKTVSKYFT KARNASGLSF
     DGNPPTFHEL RSLSARLYRN QIGDKFAQRL LGHKSDSMAA RYRDSRGREW DKIEIDK