ID   VINT_BPL54              Reviewed;         354 AA.
AC   P20709;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   23-FEB-2022, entry version 77.
DE   RecName: Full=Integrase;
DE            EC=2.7.7.- {ECO:0000250|UniProtKB:P03700};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:P03700};
GN   Name=int;
OS   Staphylococcus phage L54a (Bacteriophage L54a).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae.
OX   NCBI_TaxID=10727;
OH   NCBI_TaxID=1279; Staphylococcus.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2526804; DOI=10.1128/jb.171.8.4146-4153.1989;
RA   Ye Z.-H., Lee C.Y.;
RT   "Nucleotide sequence and genetic characterization of staphylococcal
RT   bacteriophage L54a int and xis genes.";
RL   J. Bacteriol. 171:4146-4153(1989).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination. In conjunction with
CC       excisionase, integrase is also necessary for excision of the prophage
CC       from the host genome. It may bind the DNA.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; M27965; AAA98161.1; -; Genomic_DNA.
DR   SMR; P20709; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   Pfam; PF14659; Phage_int_SAM_3; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Hydrolase; Transferase;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..354
FT                   /note="Integrase"
FT                   /id="PRO_0000197524"
FT   DOMAIN          23..106
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          138..347
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        182
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        298
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        301
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        324
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        334
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   354 AA;  41019 MW;  DDDA93EAD7E243BD CRC64;
     MFRLEEKIKE KLNNKSSSEL KTLTFHALLD EWLEYHIKTS GFKVTTLDNL KTRIKNIKKN
     SSQNLLLNKI DTKYMQTFIN ELSNVYSANQ VKRQLGHMKE AIKYAVKFYN YPNEHILNSV
     TLPKKSKTIE DIEKEEAKMY NYLEMEQVIQ IRDFILNDNN MQYRARILVA GAVEVQALTG
     MRIGELLALQ VKDVDLKNKT IAINGTIHRI KCNAGFGHKD TTKTAGSKRK IAINSRIANV
     LKKIMLENKK MQQWEPSYVD RGFIFTTCQG NPMQGSRINK RLSSAAESLN INKKVTTHTL
     RHTHISLLAE MNISLKAIMK RVGHRDEKTT IKVYTHVTEK MDRELEQKLE KLVY