ID   VINT_BPMFR              Reviewed;         333 AA.
AC   P25426;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   02-JUN-2021, entry version 73.
DE   RecName: Full=Integrase;
DE            EC=2.7.7.- {ECO:0000250|UniProtKB:P03700};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:P03700};
GN   Name=int;
OS   Mycobacterium phage FRAT1 (Mycobacteriophage FRAT1).
OC   Viruses; unclassified bacterial viruses.
OX   NCBI_TaxID=12388;
OH   NCBI_TaxID=1763; Mycobacterium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1561099; DOI=10.1093/nar/20.6.1420;
RA   Haeseleer F., Pollet J.F., Bollen A., Jacobs P.;
RT   "Molecular cloning and sequencing of the attachment site and integrase gene
RT   of the temperate mycobacteriophage FRAT1.";
RL   Nucleic Acids Res. 20:1420-1420(1992).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X63702; CAA45229.1; -; Genomic_DNA.
DR   PIR; S22643; S22643.
DR   SMR; P25426; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Hydrolase; Transferase;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..333
FT                   /note="Integrase"
FT                   /id="PRO_0000197504"
FT   DOMAIN          34..112
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          132..323
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        166
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        206
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        275
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        278
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        301
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        310
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   333 AA;  37282 MW;  F7F1832A4343D233 CRC64;
     MDAEAWLASE KRLIDNEEWT PPAEREKKAA ASAITVEEYT KKWIAERDLA GGTKDLYSTH
     ARKRIYPVLG DTPVAEMTPA LVRAWWAGMG KQYPTARRHA YNVLRAVMNT AVEDKLVSEN
     PCRIEQKAPA ERDVEALTPE ELDVVAGEVF EHYRVAVYIL AWTSLRFGEL IEIRRKDIMD
     DGETMKLRVR RGPARVGEKI VVGNTKTVRS KRPVTVPPHV AAMIRAHMAD RTKMNKGPEA
     LLVTTTRGQR LSKSAFTRSL KKGYAKIGRP DLRIHDLRAV GATLAAQAGA TTKELMVRLG
     HTTPRMAMKY QMASAARDEE IARRMSELAG ITP