ID   VINT_BPML5              Reviewed;         371 AA.
AC   P22884;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 2.
DT   23-FEB-2022, entry version 98.
DE   RecName: Full=Integrase;
DE            EC=2.7.7.- {ECO:0000250|UniProtKB:P03700};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:P03700};
GN   Name=33; Synonyms=int;
OS   Mycobacterium phage L5 (Mycobacteriophage L5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Fromanvirus.
OX   NCBI_TaxID=31757;
OH   NCBI_TaxID=1763; Mycobacterium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8459766; DOI=10.1111/j.1365-2958.1993.tb01131.x;
RA   Hatfull G.F., Sarkis G.J.;
RT   "DNA sequence, structure and gene expression of mycobacteriophage L5: a
RT   phage system for mycobacterial genetics.";
RL   Mol. Microbiol. 7:395-405(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-371.
RX   PubMed=1901654; DOI=10.1073/pnas.88.8.3111;
RA   Lee M.H., Pascopella L., Jacobs W.R. Jr., Hatfull G.F.;
RT   "Site-specific integration of mycobacteriophage L5: integration-proficient
RT   vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and
RT   bacille Calmette-Guerin.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:3111-3115(1991).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA32256.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; Z18946; CAA79409.1; -; Genomic_DNA.
DR   EMBL; M65194; AAA32256.1; ALT_INIT; Genomic_DNA.
DR   PIR; S30978; S30978.
DR   RefSeq; NP_039697.1; NC_001335.1.
DR   SMR; P22884; -.
DR   GeneID; 2942919; -.
DR   KEGG; vg:2942919; -.
DR   Proteomes; UP000002123; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008979; F:prophage integrase activity; IDA:CACAO.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Hydrolase;
KW   Reference proteome; Transferase; Viral genome integration;
KW   Virus entry into host cell.
FT   CHAIN           1..371
FT                   /note="Integrase"
FT                   /id="PRO_0000197505"
FT   DOMAIN          73..151
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          171..362
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        205
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        245
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        314
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        317
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        340
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        349
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   371 AA;  41810 MW;  D2D449CE83A15A13 CRC64;
     MARRGWGSLK TQRSGRIQAS YVNPQDGVRY YALQTYDNKM DAEAWLAGEK RLIEMETWTP
     PQDRAKKAAA SAITLEEYTR KWLVERDLAD GTRDLYSGHA ERRIYPVLGE VAVTEMTPAL
     VRAWWAGMGR KHPTARRHAY NVLRAVMNTA VEDKLIAENP CRIEQKAADE RDVEALTPEE
     LDIVAAEIFE HYRIAAYILA WTSLRFGELI ELRRKDIVDD GMTMKLRVRR GASRVGNKIV
     VGNAKTVRSK RPVTVPPHVA EMIRAHMKDR TKMNKGPEAF LVTTTQGNRL SKSAFTKSLK
     RGYAKIGRPE LRIHDLRAVG ATFAAQAGAT TKELMARLGH TTPRMAMKYQ MASEARDEAI
     AEAMSKLAKT S