ID   VINT_BPP21              Reviewed;         380 AA.
AC   P27077;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   02-DEC-2020, entry version 81.
DE   RecName: Full=Integrase;
DE            EC=2.7.7.- {ECO:0000250|UniProtKB:P03700};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:P03700};
GN   Name=int;
OS   Enterobacteria phage P21 (Bacteriophage 21) (Bacteriophage P21).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Lambdavirus.
OX   NCBI_TaxID=10711;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1715186;
RA   Baker J., Limberger R., Schneider S.J., Campbell A.;
RT   "Recombination and modular exchange in the genesis of new lambdoid
RT   phages.";
RL   New Biol. 3:297-308(1991).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination. In conjunction with
CC       excisionase, integrase is also necessary for excision of the prophage
CC       from the host genome.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; M61865; AAA32336.1; -; Genomic_DNA.
DR   SMR; P27077; -.
DR   PRIDE; P27077; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008907; F:integrase activity; IEA:InterPro.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR015094; Integrase_lambda-typ_DNA-bd_N.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   Pfam; PF09003; Arm-DNA-bind_1; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Hydrolase; Transferase;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..380
FT                   /note="Integrase"
FT                   /id="PRO_0000197520"
FT   DOMAIN          84..169
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          191..377
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        228
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        251
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        332
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        335
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        355
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        364
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   380 AA;  43902 MW;  7FCB261F03694334 CRC64;
     MAARPRSHKI SIPNLYCKLD KRTGKVYWQY KHPLSGRFHS LGTDENEAKQ VATEANTIIA
     EQRTRQILSV NERLERMKGR RSDITVTEWL DKYISIQEDR LQHNELRPNS YRQKGKPIRL
     FREHCGMQHL KDITALDIAE IIDAVKAEGH NRMAQVVRMV LIDVFKEAQH AGHVPPGFNP
     AQATKQPRNR VNRQRLSLLE WQAIFESVSR RQPYLKCGML LALVTGQRLG DICNLKFSDI
     WDDMLHITQE KTGSKLAIPL NLKCDALNIT LREVISQCRD AVVSKYLVHY RHTTSQANRG
     DQVSANTLTT AFKKAKEKCG IKWEQGTAPT FHEQRSLSER LYREQGLDTQ KLLGHKSRKM
     TDRYNDDRGK DWIIVDIKTA