ID   VINT_BPP4               Reviewed;         439 AA.
AC   P08320;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   02-DEC-2020, entry version 104.
DE   RecName: Full=Integrase;
DE            EC=2.7.7.- {ECO:0000250|UniProtKB:P36932};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:P36932};
GN   Name=int;
OS   Enterobacteria phage P4 (Bacteriophage P4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales.
OX   NCBI_TaxID=10680;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2183201; DOI=10.1093/nar/18.6.1649;
RA   Halling C., Calendar R., Christie G.E., Dale E.C., Deho G., Finkel S.,
RA   Flensburg J., Ghisotti D., Kahn M.L., Lane K.B., Lin C.-S., Lindqvist B.H.,
RA   Pierson L.S., Six E.W., Sunshine M.G., Ziermann R.;
RT   "DNA sequence of satellite bacteriophage P4.";
RL   Nucleic Acids Res. 18:1649-1649(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3119856; DOI=10.1016/0022-2836(87)90026-x;
RA   Pierson L.S., Kahn M.L.;
RT   "Integration of satellite bacteriophage P4 in Escherichia coli. DNA
RT   sequences of the phage and host regions involved in site-specific
RT   recombination.";
RL   J. Mol. Biol. 196:487-496(1987).
RN   [3]
RP   SEQUENCE REVISION.
RA   Halling C.H.;
RL   Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 430-439.
RX   PubMed=2403440; DOI=10.1128/jvi.64.1.24-36.1990;
RA   Ghisotti D., Finkel S., Halling C., Deho G., Sironi G., Calendar R.;
RT   "Nonessential region of bacteriophage P4: DNA sequence, transcription, gene
RT   products, and functions.";
RL   J. Virol. 64:24-36(1990).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X51522; CAA35897.1; -; Genomic_DNA.
DR   EMBL; X05947; CAA29379.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; M27748; AAA32430.1; -; Genomic_DNA.
DR   PIR; JW0023; RSBPP4.
DR   RefSeq; NP_042035.1; NC_001609.1.
DR   SMR; P08320; -.
DR   GeneID; 1261092; -.
DR   KEGG; vg:1261092; -.
DR   Proteomes; UP000009093; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0039695; P:DNA-templated viral transcription; IMP:CACAO.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   Gene3D; 3.30.160.390; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR038488; Integrase_DNA-bd_sf.
DR   InterPro; IPR025166; Integrase_DNA_bind_dom.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   Pfam; PF13356; Arm-DNA-bind_3; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Hydrolase;
KW   Reference proteome; Transferase; Viral genome integration;
KW   Virus entry into host cell.
FT   CHAIN           1..439
FT                   /note="Integrase"
FT                   /id="PRO_0000197507"
FT   DOMAIN          102..182
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          205..398
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        244
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        273
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        347
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        350
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        374
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        384
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   439 AA;  50839 MW;  EEB7023721395BFF CRC64;
     MCPQMKLNAR QVETAKPKDK TYKMADGGGL YLEVSAKGSK YWRMKYRRPS DKKEDRLAFG
     VWPTVTLAQA RAKRDEAKKL LVQGIDPKVV QKEARAENSG AYTFEAIARE WHASNKRWSE
     DHRSRVLRYL ELYIFPHIGS SDIRQLKTSH LLAPIKKVDA SGKHDVAQRL QQRVTAIMRY
     AVQNDYIDSN PASDMAGALS TTKARHYPAL PSSRFPEFLA RLAAYRGRVM TRIAVKLSLL
     TFVRSSELRF ARWDEFDFDK SLWRIPAKRE EIKGVRYSYR GMKMKEEHIV PLSRQAMILL
     NQLKQISGDK ELLFPGDHDA TKVMSENTVN SALRAMGYDT KTEVCGHGFR TMARGALGES
     GLWSDDAIER QLSHSERNNV RAAYIHTSEH LDERRLMMQW WADYLDMNRN KYISLMIIQN
     TKKYLNKNSY WLIFKMSVK