ID   VINT_BPPH8              Reviewed;         402 AA.
AC   P06155;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Integrase;
DE            EC=2.7.7.- {ECO:0000250|UniProtKB:P03700};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:P03700};
GN   Name=int;
OS   Enterobacteria phage phi80 (Bacteriophage phi-80).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae.
OX   NCBI_TaxID=10713;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3491212; DOI=10.1016/0022-2836(86)90491-2;
RA   Leong J.M., Nunes-Dueby S.E., Oser A.B., Lesser C.F., Youderian P.,
RA   Susskind M.M., Landy A.;
RT   "Structural and regulatory divergence among site-specific recombination
RT   genes of lambdoid phage.";
RL   J. Mol. Biol. 189:603-616(1986).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination. In conjunction with
CC       excisionase, integrase is also necessary for excision of the prophage
CC       from the host genome.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA27683.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X04051; CAA27683.1; ALT_INIT; Genomic_DNA.
DR   PIR; A24253; RSBPI8.
DR   RefSeq; YP_007947954.1; NC_021190.1.
DR   SMR; P06155; -.
DR   PRIDE; P06155; -.
DR   GeneID; 24366464; -.
DR   KEGG; vg:24366464; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   Gene3D; 3.30.160.390; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR038488; Integrase_DNA-bd_sf.
DR   InterPro; IPR025166; Integrase_DNA_bind_dom.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   Pfam; PF13356; Arm-DNA-bind_3; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Hydrolase; Transferase;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..402
FT                   /note="Integrase"
FT                   /id="PRO_0000197525"
FT   DOMAIN          102..180
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          204..390
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        242
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        269
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        338
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        341
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        364
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        373
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   402 AA;  46746 MW;  04478FFE4D6F292C CRC64;
     MCTHSMITDT KLRKALGKKR DDIEIISDSH GLNARISQAG KISFFYRYRW AGKAVKLNVG
     DYPAMSITQA RERRQQFRNW LTEGLDPREQ VKLDKQTRQE AMSVAEAFNY WIERHCIANG
     LVKVDYYRQV FEKHIAEPMK NVKVDNTAKM HWINVFDSIE SRVMAHYMLS LCKRAFRFCV
     NRSVIASNPL EGLLPSDVGQ KPKKRTRRMD DDDLRKIYQW LKSHMSIESV FLVKFIMLTG
     CRTAEIRLSE RSWFRLDDNE WVVPAGSYKT RVHIRRGLSD AAVNLVRNHL KKINTNHLVT
     SQRKIDGGIK DSPVHSPVAS NYARSIWNGT GMAEWSLHDM RRTIATNLSE LGCPPHVIEK
     LLGHQMVGVM AHYNLHDYID DQKHWLRVWQ SHLEEIIGEP FS