ID   VINT_BPSFV              Reviewed;         385 AA.
AC   P37317;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   02-DEC-2020, entry version 85.
DE   RecName: Full=Integrase;
DE            EC=2.7.7.- {ECO:0000250|UniProtKB:P36932};
DE            EC=3.1.-.- {ECO:0000250|UniProtKB:P36932};
GN   Name=int;
OS   Shigella phage Sf6 (Shigella flexneri bacteriophage VI) (Bacteriophage
OS   SfVI).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Podoviridae; Lederbergvirus.
OX   NCBI_TaxID=10761;
OH   NCBI_TaxID=623; Shigella flexneri.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1720755; DOI=10.1016/0378-1119(91)90295-m;
RA   Clark C.A., Beltrame J., Manning P.A.;
RT   "The oac gene encoding a lipopolysaccharide O-antigen acetylase maps
RT   adjacent to the integrase-encoding gene on the genome of Shigella flexneri
RT   bacteriophage Sf6.";
RL   Gene 107:43-52(1991).
CC   -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC       host genome by site-specific recombination. In conjunction with
CC       excisionase, integrase is also necessary for excision of the prophage
CC       from the host genome.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR   EMBL; X59553; CAA42131.1; -; Genomic_DNA.
DR   RefSeq; NP_958192.1; NC_005344.1.
DR   SMR; P37317; -.
DR   GeneID; 2716620; -.
DR   KEGG; vg:2716620; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.130; -; 1.
DR   Gene3D; 1.10.443.10; -; 1.
DR   Gene3D; 3.30.160.390; -; 1.
DR   InterPro; IPR044068; CB.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   InterPro; IPR038488; Integrase_DNA-bd_sf.
DR   InterPro; IPR025166; Integrase_DNA_bind_dom.
DR   InterPro; IPR010998; Integrase_recombinase_N.
DR   InterPro; IPR004107; Integrase_SAM-like_N.
DR   Pfam; PF13356; Arm-DNA-bind_3; 1.
DR   Pfam; PF14659; Phage_int_SAM_3; 1.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51900; CB; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   3: Inferred from homology;
KW   DNA integration; DNA recombination; DNA-binding; Hydrolase; Transferase;
KW   Viral genome integration; Virus entry into host cell.
FT   CHAIN           1..385
FT                   /note="Integrase"
FT                   /id="PRO_0000197508"
FT   DOMAIN          91..172
FT                   /note="Core-binding (CB)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT   DOMAIN          195..373
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        234
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        261
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        324
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        327
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        350
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        360
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   385 AA;  44103 MW;  6BA5A3431736DDB4 CRC64;
     MLTVKQIEAA KPKEKPYRLL DGNGLYLYVP VSGKKVWQLR YKIDGKEKIL TVGKYPLMTL
     QEARDKAWTA RKDISVGIDP VKAKKASSNN NSFSAIYKEW YDHKKQVWSV GYATELAKMF
     DDDILPIIGG LEIQDIEPMQ LLEVIRRFED RGAMERANKA RRRCGEVFRY AIVTGRAKYN
     PAPDLADAMK GYRKKNFPFL PADQIPAFNK ALATFSGSIV SLIATKVLRY TALRTKELRS
     MQWKNVDFEN RIITIDASVM KGRKIHVVPM SDQVIELLTT LSSITKPVSE FVFAGRNDKK
     KPICENAVLL VIKQIGYEGL ESGHGFRHEF STIMNEHEWP ADAIEVQLAH ANGGSVRGIY
     NHAQYLDKRR EMMQWWADWL DEKVR