VINT_SSV1
ID VINT_SSV1 Reviewed; 335 AA.
AC P20214;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 23-FEB-2022, entry version 99.
DE RecName: Full=Integrase;
DE EC=2.7.7.- {ECO:0000305|PubMed:22683788};
DE EC=3.1.-.- {ECO:0000305|PubMed:22683788};
GN ORFNames=d335;
OS Sulfolobus spindle-shape virus 1 (SSV1).
OC Viruses; Fuselloviridae; Alphafusellovirus.
OX NCBI_TaxID=244589;
OH NCBI_TaxID=2287; Saccharolobus solfataricus (Sulfolobus solfataricus).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1926776; DOI=10.1016/0042-6822(91)90771-3;
RA Palm P., Schleper C., Grampp B., Yeats S., McWilliam P., Reiter W.-D.,
RA Zillig W.;
RT "Complete nucleotide sequence of the virus SSV1 of the archaebacterium
RT Sulfolobus shibatae.";
RL Virology 185:242-250(1991).
RN [2]
RP FUNCTION.
RX PubMed=10430570; DOI=10.1093/genetics/152.4.1397;
RA Stedman K.M., Schleper C., Rumpf E., Zillig W.;
RT "Genetic requirements for the function of the archaeal virus SSV1 in
RT Sulfolobus solfataricus: construction and testing of viral shuttle
RT vectors.";
RL Genetics 152:1397-1405(1999).
RN [3]
RP INTEGRATION SITE.
RX PubMed=14747560; DOI=10.1128/jvi.78.4.1954-1961.2004;
RA Wiedenheft B., Stedman K., Roberto F., Willits D., Gleske A.K., Zoeller L.,
RA Snyder J., Douglas T., Young M.;
RT "Comparative genomic analysis of hyperthermophilic archaeal Fuselloviridae
RT viruses.";
RL J. Virol. 78:1954-1961(2004).
RN [4]
RP FUNCTION.
RX PubMed=22683788; DOI=10.1107/s0907444912007202;
RA Zhan Z., Ouyang S., Liang W., Zhang Z., Liu Z.J., Huang L.;
RT "Structural and functional characterization of the C-terminal catalytic
RT domain of SSV1 integrase.";
RL Acta Crystallogr. D 68:659-670(2012).
CC -!- FUNCTION: This protein may encode a site-specific integrase, which is
CC necessary for integration of the phage by site-specific recombination
CC into a tRNA gene of the host chromosome. Essential for virus infection.
CC {ECO:0000269|PubMed:10430570, ECO:0000269|PubMed:22683788}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR EMBL; X07234; CAA30211.1; -; Genomic_DNA.
DR PIR; S03212; S03212.
DR RefSeq; NP_039778.1; NC_001338.1.
DR PDB; 3UXU; X-ray; 2.71 A; A=173-335.
DR PDB; 3VCF; X-ray; 2.70 A; A/B=174-335.
DR PDB; 4DKS; X-ray; 2.70 A; A=174-335.
DR PDBsum; 3UXU; -.
DR PDBsum; 3VCF; -.
DR PDBsum; 4DKS; -.
DR SMR; P20214; -.
DR GeneID; 2559644; -.
DR KEGG; vg:2559644; -.
DR Proteomes; UP000000854; Genome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR Gene3D; 1.10.443.10; -; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR031857; Integrase_SSV1_C.
DR InterPro; IPR012922; ORF_D-335.
DR Pfam; PF16795; Phage_integr_3; 1.
DR Pfam; PF07935; SSV1_ORF_D-335; 1.
DR SUPFAM; SSF56349; SSF56349; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA integration; DNA recombination; DNA-binding; Hydrolase;
KW Reference proteome; Transferase; Viral genome integration;
KW Virus entry into host cell.
FT CHAIN 1..335
FT /note="Integrase"
FT /id="PRO_0000197509"
FT DOMAIN 97..189
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 174..329
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT REGION 62..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 211
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 281
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 314
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT HELIX 181..192
FT /evidence="ECO:0007829|PDB:3VCF"
FT HELIX 196..208
FT /evidence="ECO:0007829|PDB:3VCF"
FT HELIX 212..220
FT /evidence="ECO:0007829|PDB:3VCF"
FT HELIX 222..224
FT /evidence="ECO:0007829|PDB:3VCF"
FT STRAND 226..228
FT /evidence="ECO:0007829|PDB:3VCF"
FT STRAND 231..237
FT /evidence="ECO:0007829|PDB:3VCF"
FT STRAND 240..242
FT /evidence="ECO:0007829|PDB:3VCF"
FT STRAND 245..252
FT /evidence="ECO:0007829|PDB:3VCF"
FT HELIX 261..269
FT /evidence="ECO:0007829|PDB:3VCF"
FT HELIX 278..290
FT /evidence="ECO:0007829|PDB:3VCF"
FT HELIX 295..301
FT /evidence="ECO:0007829|PDB:3VCF"
FT STRAND 307..310
FT /evidence="ECO:0007829|PDB:3VCF"
FT HELIX 317..334
FT /evidence="ECO:0007829|PDB:3VCF"
SQ SEQUENCE 335 AA; 38860 MW; 5C7217C516A5112D CRC64;
MTKDKTRYKY GDYILRERKG RYYVYKLEYE NGEVKERYVG PLADVVESYL KMKLGVVGDT
PLQADPPGFE PGTSGSGGGK EGTERRKIAL VANLRQYATD GNIKAFYDYL MNERGISEKT
AKDYINAISK PYKETRDAQK AYRLFARFLA SRNIIHDEFA DKILKAVKVK KANADIYIPT
LEEIKRTLQL AKDYSENVYF IYRIALESGV RLSEILKVLK EPERDICGND VCYYPLSWTR
GYKGVFYVFH ITPLKRVEVT KWAIADFERR HKDAIAIKYF RKFVASKMAE LSVPLDIIDF
IQGRKPTRVL TQHYVSLFGI AKEQYKKYAE WLKGV
