ID   VIRAL_RHIRD             Reviewed;         835 AA.
AC   P07167;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Limited host range VirA protein;
DE            Short=LHR VirA;
DE            EC=2.7.13.3;
GN   Name=virA;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiA6.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A348, and A856;
RX   PubMed=3595559; DOI=10.1002/j.1460-2075.1987.tb04830.x;
RA   Leroux B., Yanofsky M.F., Winans S.C., Ward J.E., Ziegler S.F.,
RA   Nester E.W.;
RT   "Characterization of the virA locus of Agrobacterium tumefaciens: a
RT   transcriptional regulator and host range determinant.";
RL   EMBO J. 6:849-856(1987).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; X05241; CAA28868.1; -; Genomic_DNA.
DR   PIR; A27211; A27211.
DR   AlphaFoldDB; P07167; -.
DR   SMR; P07167; -.
DR   BRENDA; 2.7.13.3; 200.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR045812; DAHL.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   Pfam; PF19443; DAHL; 1.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Crown gall tumor; Kinase; Membrane;
KW   Nucleotide-binding; Phosphoprotein; Plasmid; Transferase; Transmembrane;
KW   Transmembrane helix; Two-component regulatory system.
FT   CHAIN           1..835
FT                   /note="Limited host range VirA protein"
FT                   /id="PRO_0000074894"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        261..281
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          476..698
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   DOMAIN          720..832
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   MOD_RES         479
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         770
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   835 AA;  92444 MW;  93ADBA1E2EB5F801 CRC64;
     MTWDRLLPSR KFLLAINSWY VLALVVAAIS FAVLAIGPWK NEKSIEAILT ELQSIDVDCA
     LLQRNVLRAH AGLLRNYRPL MVPLGRVRSS IANLQQLFKK ARVEDVGELS ELLARLKSSI
     NTTDAAVASF GAQNVVFEDS LATFNQSISS LLRTSDSRDL NAAKVPELGY LMLQFSFRPN
     TELALQITQS LDQLQMSTNA DKVAIQEVVR NGRVILGVLP RLNETVKLVQ ASGTFENTKK
     LQRAYLEADS LARVVEQRVR TFLGAVSVFF CFGIVILVHK LRRRTDRLAR RLDFEEVIKK
     IGVCFEDSTE TKQSLKSSAE AALGTIENFF EANQCVLGLV NVTENEIAET FSASAPPPSW
     NERRIRKIVS LVQADEHGSI FRDYPARKAS CFNEDAPGRW ALVAFKVSDR LVAVFGLRFD
     RDPVQPASSE VQLMELAAGC VSHYVVIRCK QTQRDILERR LKHAERLEAV GTLAGGIAHE
     FNNILGVILG YAMAQNILHR RTYARHYIDR INAESNRARL IIDQILALSR RRERTARPFN
     LSALVREIAP SLRVALPSEV EVDFNIQSAQ MIVEGNPLEI EQILMNLCKN AAEACIGTGR
     IEDSVYRSFV WKHKVLANGT IPAGDYILLS FEDNGGGIGQ AALPHIFEPF FRTRAQCGGT
     GLGLSTVHGH VSAMAGFVDV ISTVGRGTRF DIYLPTSAKK PVNSESFFGP EKIPLGSGEI
     VAVVEPDPVT LERYEETIAA FGYEPVGFNT FKGLTDWVLA GKEADIVLID HSSFLDGERV
     GSWVATLEKV PIIMIGQHQK NVSISADGEA SNHFLQKPVS SKALAYVVRA NIRTE